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| Product Name | DpnII Rapid Restriction Endonuclease (50 U/uL) |
| Catalog No. | IVDRM-0405 |
| Description | Recognising 5 prime ^GATC 3 prime, this rapid recombinant endonuclease digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Dam methylation fully overlapping the site blocks cleavage, as may EcoBI methylation, so DNA from Dam positive Escherichia coli resists cutting, a property often exploited to select against methylated plasmid background in mutagenesis workflows. The enzyme is supplied at a high concentration of 50 U/uL, operates in the shared rapid series buffer at 37°C, and is heat inactivated at 80°C for 20 minutes. BfuCI, MboI, Sau3AI, BscFI, Bsp143I, BssMI, BstENII, BstMBI, Kzo9I and NdeII are listed isoschizomers. This unit stores at -20°C and ships with ice packs. |
| Intended Use | Rapid digestion of unmethylated GATC sites in plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime ^GATC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dam methylation fully overlapping the site blocks cleavage; EcoBI methylation may block cleavage; isoschizomers include BfuCI, MboI, Sau3AI, Bsp143I and Kzo9I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | High concentration 50 U/uL format; methylation selectivity against Dam modified DNA |
| Concentration | 50 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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