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| Product Name | DPBS, without Calcium and Magnesium, 1x, Sterile |
| Catalog No. | ATR-CCR-0016 |
| Description | Dulbecco's Phosphate Buffered Saline formulated without calcium chloride and magnesium chloride, sterile filtered, for use as a wash buffer in cell culture procedures prior to trypsinization. |
| Intended Use | Washing adherent cell monolayers prior to trypsinization or harvesting; diluting cells for counting; preparing cell suspensions in calcium- and magnesium-free conditions where divalent cations would interfere with EDTA-based dissociation. |
| Principle / Technology | Absence of Ca2+ and Mg2+ ions facilitates cell detachment by EDTA-based dissociation reagents by preventing calcium-dependent cadherin-mediated cell-cell adhesion and magnesium-dependent integrin-mediated cell-substrate adhesion. |
| Detection Method | pH, osmolality, cell viability after wash step |
| Sample Type | All types of adherent and suspension cultured cells |
| Performance Range / Specifications | pH 7.2–7.4; osmolality 270–300 mOsm/kg; endotoxin <0.03 EU/mL |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Use at room temperature or warm to 37°C; wash cells 1–2 times before trypsinization; use 2–5 mL per T-75 flask. |
| Components / Formulation | Potassium chloride 2.67 mM, potassium phosphate monobasic 1.47 mM, sodium chloride 138 mM, sodium phosphate dibasic 8.1 mM, in cell culture grade water |
| Storage Conditions | 15–30°C |
| Shelf Life | 24 months |
| Package Specifications | 500 mL, 1 L, 6 × 500 mL, 10 L bag |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin <0.03 EU/mL, pH, osmolality, cell viability after wash |
| Key Features | Calcium and magnesium free; sterile; endotoxin tested; standard for cell washing before trypsinization; cell culture grade |
For research use only, not for clinical use.
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