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| Product Name | DNase I, Bovine Pancreas, Type IV Powder |
| Catalog No. | IVDRM-0554 |
| Description | An endonuclease isolated originally from pancreas, DNase I targets phosphodiester bonds next to pyrimidines and yields polynucleotides carrying 5 prime phosphate and 3 prime hydroxyl ends with an average product of at least a tetranucleotide. It digests single stranded DNA, double stranded DNA and chromatin at a rate influenced by histones, works best at pH 7 to 8 and depends on calcium ions, and with magnesium present it cuts either strand at random sites while manganese drives cutting of both strands at nearly identical positions. Five millimolar calcium protects the enzyme from hydrolysis. This white to light yellow powder from bovine pancreas is Type IV with purity at or above 80% by Biuret and activity of at least 2000 Kunitz Units per mg of protein, defined at 25°C and pH 5.0 as the amount giving an increase of 0.001 in A260 per mL per minute, and it suits clearing DNA from protein samples, introducing nicks for labelled base incorporation in nick translation and removing DNA contamination during extraction. |
| Intended Use | DNA clearance, nick translation labelling and removal of DNA contamination in extraction workflows |
| Principle / Technology | Calcium dependent endonucleolytic cleavage next to pyrimidines with metal dependent cutting patterns |
| Sample Type | Single stranded DNA, double stranded DNA and chromatin |
| Specificity | Type IV; inhibited by beta-mercaptoethanol, chelating agents, SDS and actin; activated by divalent metal ions |
| Reaction Conditions / Protocol | Dissolve and digest under magnesium or manganese conditions according to the required cutting pattern |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 25 mg; 100 mg; 1 g |
| Key Features | Kunitz unit activity definition; suited to nick translation labelling workflows |
| Activity / Unit Definition | At least 2000 Kunitz Units per mg protein |
| Source / Origin | Bovine pancreas |
| Readouts / Downstream Assays | DNA digestion completeness in the prepared sample |
For in vitro diagnostic use.
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