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| Product Name | DNase I, Bovine Pancreas, Type IV, Lyophilised with Calcium Chloride |
| Catalog No. | IVDRM-0553 |
| Description | An endonuclease found in many cell types and tissues, DNase I cleaves phosphodiester bonds next to pyrimidines to give polynucleotides with 5 prime phosphate and 3 prime hydroxyl ends, with an average digestion product of at least a tetranucleotide. It digests single stranded DNA, double stranded DNA and even chromatin, whose cutting rate is influenced by histones, works best at pH 7 to 8, depends on calcium ions and is stimulated by divalent metals such as magnesium, manganese, cobalt and zinc, with 5 mM calcium protecting the enzyme from autolysis. In the presence of magnesium it cuts either strand randomly, while with manganese it cuts both strands at nearly the same position. This preparation comes from bovine pancreas as a white to light brown lyophilised powder containing calcium chloride, is Type IV with protein at or above 80% by Biuret and an activity of at least 2000 Kunitz Units per mg of protein. |
| Intended Use | DNA clearance from protein samples, nick insertion for labelled base incorporation and nucleic acid extraction workflows |
| Principle / Technology | Calcium dependent endonucleolytic cleavage next to pyrimidines, with metal dependent cutting patterns |
| Sample Type | Single stranded DNA, double stranded DNA and chromatin |
| Specificity | Type IV; cuts next to pyrimidines; random single strand cutting with magnesium and same site double strand cutting with manganese |
| Reaction Conditions / Protocol | Dissolve and digest under magnesium or manganese conditions according to the required cutting pattern |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 15 KU; 10 x 15 KU |
| Key Features | Lyophilised with calcium chloride for stability; four chromatographic components A, B, C and D |
| Activity / Unit Definition | At least 2000 Kunitz Units per mg protein |
| Source / Origin | Bovine pancreas |
| Readouts / Downstream Assays | DNA digestion completeness in the prepared sample |
For in vitro diagnostic use.
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