- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | DNA Shearing Enzyme Mix for Next-Generation Sequencing Library Preparation |
| Catalog No. | ATR-SPS-0023 |
| Description | Dual-enzyme DNA fragmentation mix (endonuclease + nicking enzyme) for random, non-biased fragmentation to NGS-suitable sizes. Produces uniform fragment distributions without GC-content bias, requiring as little as 10 ng input DNA. Compatible with automation and generates 5'-phosphorylated, 3'-hydroxyl ends ready for library construction. |
| Intended Use | Enzymatic DNA fragmentation for NGS; WGS, targeted panel, metagenomic, and ChIP-seq/ATAC-seq library preparation; PacBio/Oxford Nanopore library prep. |
| Principle / Technology | DNA endonuclease introduces random double-strand breaks; nicking enzyme creates nicks converted to DSBs; balanced system eliminates sequence bias; incubation time controls fragment size (150-1,000 bp) |
| Detection Method | Mix 10 ng-1 µg DNA with enzyme mix; incubate 5-25 min at 37 °C; inactivate 65 °C 10 min; proceed to end-repair |
| Sample Type | Purified dsDNA; 10 ng-1 µg; A260/A280 1.8-2.0; free of EDTA, SDS, ethanol |
| Sensitivity / LOD | Effective from 10 ng input; uniform fragments with CV <15%; tunable 150-1,000 bp |
| Specificity | dsDNA specific; no detectable sequence/GC bias validated by NGS; RNA and ssDNA not substrates |
| Reaction Conditions / Protocol | Fragmentation: 5-25 min at 37 °C; heat kill: 10 min 65 °C; total 20-40 min |
| Components / Formulation | Fragmentation enzyme mix, reaction buffer (10×), inactivation solution, E. coli control DNA (100 ng/µL) |
| Storage Conditions | -20 °C; avoid freeze-thaw; enzymes temperature-sensitive |
| Shelf Life | 12 months at -20 °C; 3 months after first thaw |
| Package Specifications | 25, 100, 500 reactions |
| Product Form | Liquid enzyme mix in glycerol storage buffer |
| Key Features | No sonication equipment needed; zero GC-bias; tunable size; 10 ng minimum input; automation compatible; ready ends for end-repair |
| Purity | Recombinant, affinity-purified; no exonuclease/RNase/non-specific DNase; endotoxin <0.1 EU/µg |
| Concentration | As specified on product label |
| Activity / Unit Definition | Sample processing efficiency ≥90% recovery per validation protocol |
| Molecular Weight | Not applicable or as specified for enzyme components |
| Source / Origin | Synthetic and recombinant components as specified |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Cold pack -20 °C; do not thaw |
| Expiration Date / Stability | 12 months at -20 °C; 3 months after thaw; aliquot first use; max 24 hr at 4 °C |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with downstream PCR, mass spectrometry, and immunoassay workflows |
| Recommended Buffer System | Use as directed; components optimized for stated application |
| Application Notes / Precautions | Work quickly to prevent sample degradation; use appropriate protease/phosphatase inhibitors if needed |
| Batch-to-Batch Consistency | Protein precipitation efficiency and analyte recovery within ±15% of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
