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| Product Name | DNA Polymerase I (10 U/uL) |
| Catalog No. | IVDRM-0329 |
| Description | DNA polymerase I synthesises DNA complementary to a DNA or RNA template in the 5-prime to 3-prime direction while carrying dual exonuclease activities: a double-strand-specific 5-prime to 3-prime activity and a single-strand-specific 3-prime to 5-prime activity. With an approximate molecular weight of 109,000, the enzyme drives nick translation for probe labelling, supports second-strand cDNA synthesis alongside RNase H in sequencing workflows, and labels 3-prime DNA ends. |
| Intended Use | Nick translation for probe labelling; second-strand cDNA synthesis in RNA library workflows; 3-prime end labelling of DNA. |
| Principle / Technology | Template-directed 5-prime to 3-prime synthesis paired with dual-direction exonuclease processing supports translation and labelling reactions. |
| Sample Type | DNA or RNA templates with primers |
| Performance Range / Specifications | Dual 5-prime to 3-prime and 3-prime to 5-prime exonuclease activities; about 109,000 molecular weight. |
| Specificity | Compatible with nuclease partners for nick translation and second-strand reactions. |
| Reaction Conditions / Protocol | Combine enzyme with template, primers and dNTPs under the reaction conditions for the intended application. |
| Components / Formulation | DNA polymerase I in storage buffer. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 2 years |
| Package Specifications | 500 U, 2500 U, 10000 U |
| Product Form | Enzyme solution |
| Key Features | Nick translation; second-strand cDNA synthesis; end labelling. |
| Concentration | 10 U/uL |
| Activity / Unit Definition | 5-prime to 3-prime polymerisation with dual exonuclease activities |
| Molecular Weight | approximately 109,000 |
| Readouts / Downstream Assays | Labelled probes, second-strand cDNA, end-labelled DNA |
For in vitro diagnostic use.
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