- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | DMSO (Cell Culture Grade) |
| Catalog No. | RESK-0252 |
| Description | DMSO (dimethyl sulfoxide) is an aprotic polar solvent commonly used in chemical reactions, PCR reactions, and as a vitrification cryoprotectant for the preservation of cells, tissues and organs. In cell freezing medium, DMSO protects cells from mechanical damage caused by ice crystals. It is suitable for the cryopreservation of primary cultures, subcultures, recombinant heteroploid and hybridoma cell lines, embryonic stem cells (ESC) and hematopoietic stem cells, and is often used in combination with BSA or FBS. Solubility: DMSO mixes readily with water (1 mL DMSO + 1 mL H2O) to form a clear, colorless solution. It is hygroscopic and should not be exposed to humid environments. It is also soluble in ethanol, acetone, diethyl ether, benzene and chloroform. Storage/stability: DMSO readily supercools and melts slowly at room temperature; the product may arrive as a solid rather than a liquid. Solidified DMSO can be re-liquefied at room temperature without damage. It is a thermally stable compound, remaining stable in acidic, alkaline and neutral solutions up to 100 °C, and remains stable in alkaline and neutral solutions near its boiling point (189 °C). Heating at 150 °C for 24 h results in less than 0.1% purity loss. |
| Intended Use | Cell culture grade DMSO for dissolving drugs and reagents in cell culture experiments; as the main component of cell freezing medium to protect cells from freeze-thaw damage; for dissolving and diluting bioactive molecules in biochemistry; and for drug dissolution and delivery in pharmacological studies of cell permeability and bioactivity. For research use only. |
| Principle / Technology | As a cryoprotectant, DMSO penetrates cells and reduces ice crystal formation during freezing, protecting cells from mechanical damage caused by ice crystals. |
| Sample Type | Human and animal cell lines, hybridomas, embryonic stem cells, hematopoietic stem cells, bacteriophage lambda |
| Performance Range / Specifications | Thermally stable: stable in acidic, alkaline and neutral solutions up to 100 °C; stable in alkaline and neutral solutions near its boiling point (189 °C); purity loss <0.1% after heating at 150 °C for 24 h. Boiling point: 189 °C. |
| Reaction Conditions / Protocol | 1. Cell cryopreservation procedure: 1) Prepare freezing medium containing cell culture medium, 10–20% serum and 5–10% DMSO; 2) Detach adherent cells with trypsin or another suitable method (cells should preferably be in logarithmic growth phase); 3) Collect cells by low-speed centrifugation (4 °C, 250 × g, 10 min) and aspirate the medium; 4) Resuspend cells in freezing medium at a density of 10E6–10E7 cells/mL; 5) Distribute cells evenly into cryovials; 6) Freeze cells following a standard freezing program and store at -70 °C or lower. 2. For cell fusion, add 10% DMSO to 40–50% PEG solution. 3. DMSO has been reported as a buffer component for: purification of Poly(A)+ RNA, transformation of E. coli competent cells, PCR reactions, amplification of cDNA libraries, DNA sequencing, DEAE-dextran-mediated cell transfection, and polybrene-mediated DNA transfection. 4. Procedures have been described for recovering DNA after membrane filtration using DMSO for subsequent PCR amplification. 5. For capillary electrophoresis used in DNA sequencing, 5% DMSO (v/w) is mixed into 2 M urea; the DMSO concentration can be adjusted up to 100% if needed. 6. Studies have reported the relationship between DMSO concentration and the melting temperature of oligonucleotides. 7. Studies have described using DMSO to increase monoclonal antibody production in hybridoma cells. 8. DMSO has been reported for modification of phosphoserine and phosphothreonine residues in proteins for mass spectrometry (MS) analysis of phosphorylation status. |
| Components / Formulation | Dimethyl sulfoxide (DMSO), cell culture grade. CAS No.: 67-68-5. |
| Storage Conditions | Store at room temperature. DMSO is hygroscopic; avoid exposure to humid environments. The product may solidify upon receipt; re-liquefy at room temperature without damage. |
| Shelf Life | 2 years |
| Package Specifications | 100 mL |
| Product Form | Liquid (may solidify at low temperatures; melting point 18.5 °C) |
| Purity | Cell culture grade; purity loss <0.1% after heating at 150 °C for 24 h |
| Concentration | Typically used at 5–10% in cell freezing medium; 10% in 40–50% PEG solution for cell fusion |
| Molecular Weight | 78.13 g/mol (DMSO, CAS 67-68-5) |
| Source / Origin | Synthetic |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | 2 years when stored at room temperature. Thermally stable: stable up to 100 °C in acidic, alkaline and neutral solutions; <0.1% purity loss after 150 °C for 24 h. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Fully compatible: LDPE, HDPE, polypropylene, PPCO, polymethylpentene, nylon, Teflon FEP Moderately compatible: polystyrene, ECTFE/ETFE Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate Miscible with water, ethanol, acetone, diethyl ether, benzene and chloroform. |
| Application Notes / Precautions | DMSO can be filtered, but a filter membrane suitable for organic solvents must be selected, as DMSO will dissolve ordinary membranes. DMSO readily supercools and may arrive as a solid; re-liquefy at room temperature before use. Hygroscopic; keep tightly closed and avoid humid environments. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
