- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | DMEM/F12 Medium (1:1), with L-Glutamine, HEPES, Sodium Pyruvate and Phenol Red |
| Catalog No. | RERE-0209 |
| Description | DMEM/F-12 medium, a one-to-one blend of Dulbecco's Modified Eagle Medium and Ham's F-12 Nutrient Mixture, formulated with L-glutamine, HEPES, sodium pyruvate and phenol red. The blend layers the richer trace-element and nutrient content of F-12 onto the amino-acid depth of DMEM, giving a base widely used for MDCK, glial cells, fibroblasts and human endothelial cells, among other mammalian lines. It is also a standard starting point for developing serum-free formulations and suits low-serum and cloning-density culture. HEPES holds pH steady against incubator excursions, pyruvate provides an alternative carbon source and L-glutamine supplies nitrogen-intensive nutrition. Unopened bottles keep for 12 months at 2-8°C. |
| Intended Use | Broad mammalian culture including MDCK, glial, fibroblast and endothelial lines; base for serum-free development, low-serum and cloning-density work |
| Principle / Technology | DMEM amino-acid depth combined with F-12 trace nutrients, buffered by HEPES, supports demanding lines and cloning-density growth |
| Specificity | Sterile 1x liquid; D-glucose, phenol red, L-glutamine, sodium pyruvate and HEPES present; 12 month shelf life |
| Reaction Conditions / Protocol | Use directly under aseptic technique; supplement with serum or serum-free additives the workflow requires and equilibrate to 37°C before inoculation |
| Storage Conditions | 2-8°C |
| Package Specifications | 500 mL | 10 x 500 mL |
| Key Features | 1:1 DMEM and F-12 blend; HEPES buffered with pyruvate as alternate carbon; 12 month shelf life |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
