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| Product Name | DMEM/F12 50:50 Mixture, with L-Glutamine and HEPES |
| Catalog No. | ATR-CCR-0003 |
| Description | A 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F12 nutrient mixture, combining the rich nutrient profile of DMEM with the broad supplement range of F12 for serum-reduced culture of diverse cell types. |
| Intended Use | Culture of primary cells, stem cells, and cell lines under reduced serum conditions; commonly used as the basal medium for serum-free formulations and specialized cell types including prostate epithelium, mammary epithelium, and pancreatic cells. |
| Principle / Technology | DMEM provides high concentrations of amino acids, vitamins, and glucose; Ham's F12 supplements trace elements, putrescine, linoleic acid, and additional vitamins; the combination supports cells with complex nutritional requirements. |
| Detection Method | Growth curve analysis, colony formation efficiency, differentiation marker expression |
| Sample Type | Primary epithelial cells, stem cells, prostate and mammary cell lines, keratinocytes, melanocytes |
| Performance Range / Specifications | Supports growth in 2–5% serum (versus 10% for standard DMEM); equivalent or better growth for primary cells than either medium alone |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Supplement according to cell type: typically 5–10% FBS for cell lines, reduced serum or serum-free supplements for primary cells; culture at 37°C in 5% CO2. |
| Components / Formulation | 1:1 blend of DMEM and Ham's F12, 2.5 mM L-glutamine, 15 mM HEPES, sodium bicarbonate, phenol red |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin, pH, osmolality, cell growth promotion with primary and established cell lines |
| Key Features | Nutrient-rich 1:1 blend; supports serum-reduced culture; HEPES buffered; with L-glutamine; ideal base for serum-free formulations |
For research use only, not for clinical use.
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