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| Product Name | DMEM/F-12 Reduced Serum Medium |
| Catalog No. | RESK-0644 |
| Description | DMEM/F-12 Reduced Serum Medium is based on the classical DMEM/F-12 formulation and is additionally supplemented with ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin, and trace elements. It sustains cell growth under low-serum conditions and is intended for reduced-serum mammalian cell culture. Compared with classical DMEM/F-12, this formulation reduces serum consumption by 50-90% without affecting cell growth rate or morphology, thereby minimizing variability in culture conditions and extending the usable life of a single serum lot. |
| Intended Use | For reduced-serum mammalian cell culture, supporting cell growth under low-serum conditions. |
| Principle / Technology | Enriched with defined supplements (ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin, and trace elements) on the classical DMEM/F-12 base, the medium maintains cell growth while substantially lowering the serum requirement. |
| Sample Type | Mammalian cells, including MRC-5, SP2, Vero, WI-38, and Jurkat cells. |
| Performance Range / Specifications | Reduces serum usage by 50-90% compared with classical DMEM/F-12 without affecting cell growth rate or morphology. |
| Reaction Conditions / Protocol | Before use, supplement with 1-5% fetal bovine serum and 4 mM L-glutamine or alanyl-glutamine. Because different cell types differ in their dependence on serum, optimization for each cell type is recommended to determine the final serum concentration. |
| Components / Formulation | Classical DMEM/F-12 base supplemented with ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin, and trace elements. Prepared with water for injection. With (+) phenol red. Without (-) L-glutamine and HEPES. |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year. |
| Package Specifications | 500 mL |
| Product Form | Liquid |
| Expiration Date / Stability | Stable for 1 year when stored at 2-8 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for the culture of MRC-5, SP2, Vero, WI-38, and Jurkat cells; applicable to reduced-serum mammalian cell culture. |
| Application Notes / Precautions | This medium contains no L-glutamine or HEPES; add 1-5% fetal bovine serum and 4 mM L-glutamine or alanyl-glutamine before use. Serum dependence varies among cell types; optimize the final serum concentration for each cell line. |
For research use only, not for clinical use.
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