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| Product Name | DMEM/F-12 Medium (with L-Glutamine, Sodium Pyruvate, Phenol Red and HEPES) |
| Catalog No. | RESK-0636 |
| Description | DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Ham) is a 1:1 mixture of DMEM medium and Ham's F-12 medium. It supports the growth of a variety of mammalian cells, including glial cells, fibroblasts, MDCK cells, human endothelial cells and rat fibroblasts. DMEM is a modification of Eagle's Basal Medium (BME) with four times the vitamin and amino acid concentrations of BME, while Ham's F-12 is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine and several amino acids. L-Glutamine is an essential nutrient in the cell culture liquid environment, and sodium pyruvate can serve as an alternative carbon source in cell culture, allowing cells to metabolize sodium pyruvate when glucose is insufficient. This product contains: L-Glutamine, Sodium Pyruvate, Phenol Red, and HEPES. |
| Intended Use | Basal medium providing the nutrients and buffering system required for cell growth; supports the in vitro culture and proliferation of various mammalian cells, including glial cells, fibroblasts, MDCK cells, human endothelial cells and rat fibroblasts. |
| Principle / Technology | A 1:1 mixture of DMEM and Ham's F-12 medium combining the enriched vitamin and amino acid profile of DMEM with the elevated choline, inositol, putrescine and amino acid concentrations of Ham's F-12; supplemented with L-Glutamine and sodium pyruvate, and buffered with HEPES in addition to the standard buffering system. |
| Sample Type | Mammalian cells, including glial cells, fibroblasts, MDCK cells, human endothelial cells and rat fibroblasts; applicable species: human, rat, dog. |
| Performance Range / Specifications | Medium type: DMEM/F-12 (1:1 mixture of DMEM and Ham's F-12) Contains: L-Glutamine, Sodium Pyruvate, Phenol Red, HEPES |
| Reaction Conditions / Protocol | Use under standard mammalian cell culture conditions (supplement with serum and antibiotics as required by the specific cell type). |
| Components / Formulation | 1:1 mixture of DMEM and Ham's F-12 medium; contains L-Glutamine, Sodium Pyruvate, Phenol Red and HEPES. |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year. |
| Package Specifications | 500 mL. |
| Product Form | Liquid. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Supports the growth of a variety of mammalian cells, including glial cells, fibroblasts, MDCK cells, human endothelial cells and rat fibroblasts. |
| Recommended Buffer System | Contains HEPES buffer. |
| Application Notes / Precautions | Store at 2-8 °C protected from light; L-Glutamine is unstable in aqueous solution. |
For research use only, not for clinical use.
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