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| Product Name | DMEM High Glucose Medium (with L-Glutamine and Sodium Pyruvate; without HEPES and Antibiotics) |
| Catalog No. | RESK-0631 |
| Description | DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium suitable for the culture of many mammalian cells, including primary fibroblasts, neurons, glial cells, HUVEC and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7 and PC-12. Developed on the basis of MEM medium, DMEM contains twice the amino acids and four times the vitamins of MEM, with additional non-essential amino acids, trace iron ions and sodium pyruvate. DMEM was originally designed as a low-glucose formulation containing 1000 mg/L glucose, and the high-glucose version containing 4500 mg/L glucose was later developed and is now widely used for the culture of various cells. High-glucose DMEM is commonly used for fast-growing, low-adherence cells, hybridoma myeloma cells, cloned cells, DNA-transfected transformed cells, primary virus host cells, single-cell culture, and vaccine production, such as the expression of EPO in CHO cells and the production of hepatitis B vaccine. L-Glutamine is an essential nutrient in the cell culture liquid environment but is unstable in aqueous solution and degrades readily. Sodium pyruvate can serve as an alternative carbon source in cell culture, allowing cells to metabolize sodium pyruvate when glucose is insufficient. This product contains: D-Glucose (4500 mg/L), Phenol Red, L-Glutamine, and Sodium Pyruvate. This product does not contain: HEPES or antibiotics. |
| Intended Use | Basal medium for the in vitro culture of a wide range of mammalian cells, including primary fibroblasts, neurons, glial cells, HUVEC, smooth muscle cells, and cell lines such as HeLa, 293, Cos-7 and PC-12; suitable for fast-growing, low-adherence cells, hybridoma myeloma cells, cloned cells, DNA-transfected transformed cells, primary virus host cells, single-cell culture, and vaccine production. |
| Principle / Technology | Provides the amino acids, vitamins, inorganic salts, glucose and other nutrients required for mammalian cell growth in vitro; sodium pyruvate serves as an alternative carbon source when glucose is limiting. |
| Sample Type | Mammalian cells (adherent and suspension cells, primary cells and cell lines). |
| Performance Range / Specifications | D-Glucose: 4500 mg/L Contains: L-Glutamine, Sodium Pyruvate (110 mg/L), Phenol Red Without: HEPES and antibiotics |
| Reaction Conditions / Protocol | Use under standard mammalian cell culture conditions (supplement with serum and antibiotics as required by the specific cell type). |
| Components / Formulation | Amino Acids Glycine: 30.0 mg/L (0.4 mM) L-Arginine hydrochloride: 84.0 mg/L (0.398 mM) L-Cystine 2HCl: 63.0 mg/L (0.201 mM) L-Glutamine: 584.0 mg/L (4.0 mM) L-Histidine hydrochloride-H2O: 42.0 mg/L (0.2 mM) L-Isoleucine: 105.0 mg/L (0.802 mM) L-Leucine: 105.0 mg/L (0.802 mM) L-Lysine hydrochloride: 146.0 mg/L (0.798 mM) L-Methionine: 30.0 mg/L (0.201 mM) L-Phenylalanine: 66.0 mg/L (0.4 mM) L-Serine: 42.0 mg/L (0.4 mM) L-Threonine: 95.0 mg/L (0.798 mM) L-Tryptophan: 16.0 mg/L (0.078 mM) L-Tyrosine disodium salt dihydrate: 104.0 mg/L (0.398 mM) L-Valine: 94.0 mg/L (0.803 mM) Vitamins Choline chloride: 4.0 mg/L (0.029 mM) D-Calcium pantothenate: 4.0 mg/L (0.008 mM) Folic Acid: 4.0 mg/L (0.009 mM) Niacinamide: 4.0 mg/L (0.033 mM) Pyridoxine hydrochloride: 4.0 mg/L (0.019 mM) Riboflavin: 0.4 mg/L (0.001 mM) Thiamine hydrochloride: 4.0 mg/L (0.012 mM) i-Inositol: 7.2 mg/L (0.04 mM) Inorganic Salts Calcium Chloride (CaCl2, anhyd.): 200.0 mg/L (1.802 mM) Ferric Nitrate (Fe(NO3)3-9H2O): 0.1 mg/L (0.00025 mM) Magnesium Sulfate (MgSO4, anhyd.): 97.67 mg/L (0.814 mM) Potassium Chloride (KCl): 400.0 mg/L (5.333 mM) Sodium Chloride (NaCl): 6400.0 mg/L (110.345 mM) Sodium Phosphate monobasic (NaH2PO4-H2O): 125.0 mg/L (0.906 mM) Sodium Bicarbonate (NaHCO3): 3700.0 mg/L (44.0 mM) Other Components D-Glucose (Dextrose): 4500.0 mg/L (25.0 mM) Phenol Red: 15.0 mg/L (0.04 mM) Sodium Pyruvate: 110.0 mg/L (1.0 mM) |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year. |
| Package Specifications | 500 mL. |
| Product Form | Liquid. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for the culture of primary fibroblasts, neurons, glial cells, HUVEC, smooth muscle cells, HeLa, 293, Cos-7, PC-12 cells, hybridoma myeloma cells, cloned cells, DNA-transfected transformed cells, primary virus host cells, and CHO cells. |
| Application Notes / Precautions | L-Glutamine is unstable in aqueous solution and degrades readily; store at 2-8 °C protected from light. |
For research use only, not for clinical use.
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