- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | DMEM (Dulbecco's Modified Eagle Medium), High Glucose, with L-Glutamine |
| Catalog No. | ATR-CCR-0001 |
| Description | A high-glucose (4.5 g/L) formulation of Dulbecco's Modified Eagle Medium containing 4 mM L-glutamine, optimized for the growth of a broad range of mammalian cell lines with elevated metabolic requirements. |
| Intended Use | Routine culture of adherent and suspension mammalian cell lines including HeLa, HEK293, COS-7, NIH/3T3, and many cancer cell lines; suitable for transfection experiments requiring high cell density. |
| Principle / Technology | High glucose concentration supports rapid proliferation of cells with glycolytic metabolism; sodium bicarbonate/CO2 buffering system maintains physiological pH in 5–10% CO2 atmosphere; enriched with 4-fold higher amino acid and vitamin concentrations compared to basal MEM. |
| Detection Method | Cell proliferation assays, morphology assessment by phase-contrast microscopy, mycoplasma testing |
| Sample Type | Adherent and suspension mammalian cell lines |
| Performance Range / Specifications | Supports doubling times of 18–24 hours for common cell lines; pH 7.0–7.4 at equilibrium in 5% CO2 |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Supplement with 10% FBS and 1% penicillin-streptomycin before use; warm to 37°C; culture in 5% CO2 humidified incubator; passage at 70–90% confluence. |
| Components / Formulation | DMEM base with 4.5 g/L glucose, 4 mM L-glutamine, sodium pyruvate, phenol red pH indicator, sodium bicarbonate buffer system |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | 500 mL, 1 L, 6 × 500 mL, 10 L bag |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin <0.25 EU/mL, pH 7.0–7.4, osmolality 320–350 mOsm/kg, cell growth promotion testing with reference cell lines |
| Key Features | High glucose formulation; with L-glutamine; standard for many cell lines; sterile and endotoxin tested; consistent lot performance |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
