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| Product Name | DH5α Chemically Competent Cells |
| Catalog No. | ATR-CCR-0046 |
| Description | Chemically competent DH5α cells for routine plasmid cloning, propagation and blue-white screening. |
| Intended Use | Plasmid transformation; gene cloning; blue-white screening; transformation of PCR products, cDNA and other non-methylated DNA. |
| Principle / Technology | The cloning strain combines recA1 and endA1 mutations to improve recombinant plasmid stability and support high-quality plasmid preparation, with lacZΔM15 for blue-white screening. |
| Sample Type | Plasmid DNA; PCR products; cDNA; other non-methylated DNA. |
| Performance Range / Specifications | Transformation efficiency at least 10⁸ cfu/µg pUC19 DNA. |
| Specificity | Suitable for high-efficiency uptake of common cloning plasmids and non-methylated DNA. |
| Components / Formulation | Aliquoted chemically competent DH5α Escherichia coli cell suspension. |
| Storage Conditions | Store at −85 to −65°C. |
| Package Specifications | 10 × 100 µL; 20 × 100 µL. |
| Product Form | Chemically competent E. coli cloning cells. |
| Quality Control | Transformation efficiency is qualified at ≥10⁸ cfu/µg pUC19 DNA. |
| Key Features | High transformation efficiency; stable maintenance of foreign DNA; supports blue-white screening; suitable for high-quality plasmid preparation. |
| Compatibility | Compatible with routine cloning vectors and blue-white screening workflows. |
| Application Notes / Precautions | Keep frozen until use and minimize freeze–thaw cycles. |
| Genotype | deoR endA1 gyrA96 hsdR17 (rk− mk+) recA1 relA1 supE44 thi-1 Δ(lacZYA-argF)U169 Φ80lacZΔM15 F− λ− |
For research use only, not for clinical use.
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