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| Product Name | Density Gradient Medium (Iodixanol Solution, 60% w/v) |
| Catalog No. | RESK-0259 |
| Description | Iodixanol (molecular formula C35H44I6N6O15, CAS No. 92339-11-2) is a non-ionic, hydrophilic compound. This product is a 60% (w/v) aqueous iodixanol solution - a sterile, endotoxin-tested, low-osmolarity, non-ionic iodinated density gradient medium designed for the in vitro separation of biological particles. It is commonly used to separate macromolecules, viruses, cells and subcellular organelles/membranes, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes and natural killer cells, and is also widely used in medicine as a contrast agent. It is broadly applicable to separations that demand high resolution, high viability and high recovery, covering cells (including sensitive cells), organelles, membrane vesicles, viruses and dead cells. |
| Intended Use | In vitro separation of biological particles by density gradient centrifugation Isolation of macromolecules (e.g., DNA, cytokines), viruses, cells and subcellular organelles/membranes, including lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes and natural killer cells Separation scenarios requiring high resolution, high viability and high recovery, including sensitive cells, membrane vesicles and dead cells |
| Principle / Technology | Density gradient centrifugation using a non-ionic iodinated medium; both continuous and discontinuous gradients can be formed. The medium is iso-osmotic at useful densities and has low viscosity and no cytotoxicity. |
| Detection Method | Density gradient centrifugation (continuous or discontinuous gradients). |
| Sample Type | Cells (including sensitive cells), subcellular organelles and membranes, membrane vesicles, viruses, macromolecules (e.g., DNA, cytokines) and dead cells. |
| Performance Range / Specifications | Concentration: 60% (w/v) iodixanol in water Sterile and endotoxin-tested Non-ionic, hydrophilic iodinated compound with low osmolarity; iso-osmotic at useful densities Low viscosity; no cytotoxicity Gradient type: suitable for both continuous and discontinuous gradients |
| Components / Formulation | 60% (w/v) iodixanol aqueous solution; sterile and endotoxin-tested. |
| Storage Conditions | Store at room temperature, protected from light; 4-24 °C is optimal. |
| Shelf Life | 3 years. |
| Package Specifications | 500 mL 250 mL |
| Product Form | Sterile liquid solution |
| Quality Control | Sterile; endotoxin-tested. |
| Concentration | 60% (w/v) |
| Molecular Weight | 1550.18 g/mol (calculated from the molecular formula C35H44I6N6O15) |
| Source / Origin | CAS No. 92339-11-2; molecular formula C35H44I6N6O15. |
| Expiration Date / Stability | Stable for 3 years when stored protected from light at room temperature (4-24 °C optimal). |
| Compatibility | Residual iodixanol does not need to be removed before common downstream analyses (e.g., electrophoresis); removal is only required before electron microscopy. |
| Application Notes / Precautions | Gentle and non-toxic: non-ionic with low osmotic pressure; does not cause cell damage or apoptosis. Metabolically inert: does not interfere with normal cellular metabolism or the determination of marker enzymes. Store protected from light; 4-24 °C is optimal. |
For research use only, not for clinical use.
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