dCas9 Nuclease, Catalytically Dead SpCas9 (10 mg/mL)

Name: dCas9 Nuclease, Catalytically Dead SpCas9 (10 mg/mL)
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Cat.No: IVDRM-0585 Datasheet

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Product Name	dCas9 Nuclease, Catalytically Dead SpCas9 (10 mg/mL)
Catalog No.	IVDRM-0585
Description	Derived from Streptococcus pyogenes SpCas9, dCas9 Nuclease carries D10A and H840A substitutions that completely inactivate the HNH and RuvC nuclease domains. The protein keeps its sgRNA-guided ability to bind target DNA specifically but has no DNA cleavage activity and creates no double-strand break, acting as a catalytically dead DNA binding platform. Fusion of effector domains such as KRAB, VP64, fluorescent proteins or methyltransferases to dCas9 enables targeted regulation of chosen genes or loci, supporting CRISPR interference, CRISPR activation, epigenetic editing and genome imaging. Purity is at least 95% with negative endonuclease, exonuclease and RNase activity and endotoxin below 10 EU per mg.
Intended Use	CRISPR interference, CRISPR activation, epigenetic editing and genome imaging using a DNA binding platform
Principle / Technology	D10A and H840A inactivation of both nuclease domains while sgRNA-guided DNA binding is preserved
Sample Type	Cells and DNA targets addressed by sgRNA-guided dCas9 effector fusions
Specificity	No DNA cleavage activity and no double-strand break; retains specific target DNA binding
Reaction Conditions / Protocol	Deliver the dCas9 effector complex by transfection and read out the targeted regulation
Storage Conditions	-25 to -15°C
Shelf Life	2 years
Package Specifications	100 ug; 1 mg
Key Features	Catalytically dead DNA binding platform; compatible with a range of effector domain fusions
Purity	Greater than or equal to 95%
Concentration	10 to 12 mg/mL
Source / Origin	Streptococcus pyogenes
Readouts / Downstream Assays	Reporter or transcriptional readout at the targeted locus

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For in vitro diagnostic use.