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| Product Name | CT26 Human DLL3 & CD47 Stable Cell Line |
| Catalog No. | ATR-CCR-0077 |
| Description | A cryopreserved, adherent CT26 cell line stably expressing tag-free human DLL3 & CD47. The model is maintained under puromycin and blasticidin selection for cell-based target-binding studies. |
| Intended Use | For FACS binding assays, cell-based ELISA and other target-specific cell research applications. |
| Principle / Technology | Stable transfection followed by antibiotic selection; DLL3 & CD47 expression is qualified by flow cytometry. |
| Detection Method | Flow cytometry; cell-based ELISA |
| Sample Type | Stable mammalian cell line |
| Performance Range / Specifications | Approximately 5 x 10^6 cells in 1 mL per vial; adherent growth. |
| Specificity | Human DLL3 and CD47 |
| Reaction Conditions / Protocol | Culture in RPMI 1640 with 10% FBS, 10 ug/mL puromycin and 8 ug/mL blasticidin. |
| Components / Formulation | Cryopreservation medium: 35% FBS, 55% RPMI 1640 and 10% DMSO. |
| Storage Conditions | Store frozen in liquid nitrogen or at -80 C. |
| Package Specifications | 1 vial; approximately 5 x 10^6 cells in 1 mL |
| Product Form | Cryopreserved stable cell line |
| Quality Control | CD47 and DLL3 co-expression verified separately by flow cytometry. Cells were stained with anti-CD47 and anti-DLL3 antibodies at 2 ug per 1 x 10^6 cells and evaluated using FITC and APC fluorescence channels, respectively. |
| Key Features | Stable human DLL3 & CD47 co-expression; adherent growth; puromycin and blasticidin resistance; tag-free; qualified for FACS and cell-based ELISA applications. |
| Source / Origin | CT26 cells stably expressing human DLL3 & CD47. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | FACS binding assays; cell-based ELISA |
| Recommended Buffer System | RPMI 1640 with 10% FBS, 10 ug/mL puromycin and 8 ug/mL blasticidin |
| Application Notes / Precautions | Maintain puromycin and blasticidin selection during routine culture. Keep the vial frozen until cell recovery and use aseptic cell-culture technique. |
| Genotype | Stable human DLL3 & CD47 co-expression; puromycin and blasticidin-resistant. |
| Alternative Names / Synonyms | DLL3 and CD47; Delta-like protein 3 and Leukocyte surface antigen CD47 |
| Lead Time | 1-3 working days |
| Host Species | Human |
| Tag Information | Tag-Free |
| UniProt Accession | Q08722 (DLL3) |
For research use only, not for clinical use.
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