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CT26 Human BTNL10 Stable Cell Line

Cat.No: ATR-CCR-0075 Datasheet

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Product Name CT26 Human BTNL10 Stable Cell Line
Catalog No. ATR-CCR-0075
Description A cryopreserved, adherent CT26 cell line stably expressing n-terminal flag-tagged human BTNL10. The model is maintained under puromycin selection for cell-based target-binding studies.
Intended Use For FACS binding assays, cell-based ELISA and other target-specific cell research applications.
Principle / Technology Stable transfection followed by antibiotic selection; BTNL10 expression is qualified by flow cytometry.
Detection Method Flow cytometry; cell-based ELISA
Sample Type Stable mammalian cell line
Performance Range / Specifications Approximately 5 x 10^6 cells in 1 mL per vial; adherent growth.
Specificity Human BTNL10
Reaction Conditions / Protocol Culture in RPMI 1640 with 10% FBS and 1 ug/mL puromycin.
Components / Formulation Cryopreservation medium: 35% FBS, 55% RPMI 1640 and 10% DMSO.
Storage Conditions Store frozen in liquid nitrogen or at -80 C.
Package Specifications 1 vial; approximately 5 x 10^6 cells in 1 mL
Product Form Cryopreserved stable cell line
Quality Control Target expression verified by anti-Flag staining followed by flow-cytometric analysis.
Key Features Stable human BTNL10 expression; adherent growth; puromycin resistance; n-terminal flag-tagged; qualified for FACS and cell-based ELISA applications.
Source / Origin CT26 cells stably expressing human BTNL10.
Regulatory / Compliance For research use only; not for clinical diagnosis or treatment.
Compatibility FACS binding assays; cell-based ELISA
Recommended Buffer System RPMI 1640 with 10% FBS and 1 ug/mL puromycin
Application Notes / Precautions Maintain puromycin selection during routine culture. Keep the vial frozen until cell recovery and use aseptic cell-culture technique.
Genotype Stable human BTNL10 expression; puromycin-resistant.
Alternative Names / Synonyms BTNL10; Butyrophilin-like protein 10
Lead Time 1-3 working days
Host Species Human
Tag Information N-terminal Flag-tagged
UniProt Accession A8MVZ5

For research use only, not for clinical use.

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