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| Product Name | Collagen Type I (from Rat Tail) |
| Catalog No. | RESK-0201 |
| Description | Collagen is the major component of the extracellular matrix in connective tissues and visceral organs, and is most abundant in skin, tendon, and bone. Type I collagen is a 300 nm heterotrimer composed of two alpha1 chains and one alpha2 chain. When used as a gel, collagen supports in vitro cell culture and enhances the expression of cell-specific morphology and functions. It can also be applied as a thin coating to promote cell attachment. Applications include tumor cell invasion and migration studies, culture or differentiation studies of monocytes and macrophages, autoradiographic studies of granulocytes and macrophages, and research on maintaining hepatocyte function, differentiation status, and elevated gene transcription levels. This product is derived from Sprague-Dawley (SD) rat tail, dissolved in 0.02 M acetic acid, with purity greater than 90% determined by SDS-PAGE, and is sterile. |
| Intended Use | For research use in 3D gel cell culture, cell surface coating to promote attachment, tumor cell invasion and migration assays, monocyte/macrophage culture and differentiation studies, and hepatocyte function maintenance studies. |
| Principle / Technology | Acid-solubilized native Type I collagen neutralized to physiological pH polymerizes into a fibrillar gel, providing an extracellular matrix scaffold that supports cell adhesion, morphology, and function in 2D coating and 3D culture systems. |
| Sample Type | Cultured cells (e.g., tumor cells, monocytes, macrophages, hepatocytes) for coating or 3D gel embedding. |
| Performance Range / Specifications | Manufactured in a cleanroom to ensure cleanliness Sourced from SD rat tail Single-batch yield up to 3 L to support large-scale use Validated by cell culture testing (including 3D gel cell culture) Protein purity >90% by SDS-PAGE Sterile solution, free of mycoplasma contamination |
| Reaction Conditions / Protocol | 3D gel preparation (example: 1 mL of 1 mg/mL gel): Add 200 uL collagen stock solution (5 mg/mL) to a pre-chilled tube on ice, then add 695 uL pre-chilled sterile water and mix. Add the diluted collagen into a tube containing 4.6 uL of 1 M NaOH. Follow this order of addition strictly, otherwise gelation will fail. Dispense into culture plates, incubate at 37 °C for 30 min to solidify, then add an appropriate volume of culture medium. Coating method: Dilute with 0.02 M acetic acid to the desired concentration, add the appropriate volume to the wells, incubate at room temperature for 1 h, aspirate excess liquid, wash 3-5 times with PBS, and use directly. For 3D culture, a final collagen concentration of 1-2 mg/mL is recommended. |
| Components / Formulation | Type I collagen dissolved in 0.02 M acetic acid (HAc) solution; sterile. |
| Storage Conditions | Store at 2-8 °C. Do not freeze. |
| Shelf Life | 1 year when stored at 2-8 °C. |
| Package Specifications | 2 mL 10 mL |
| Product Form | Sterile liquid solution |
| Quality Control | Purity >90% verified by SDS-PAGE; sterility tested; mycoplasma-free; validated by cell culture testing including 3D gel cell culture. |
| Purity | >90% (SDS-PAGE) |
| Concentration | Stock concentration is approximately 5 mg/mL (batch-dependent; e.g., a recent lot measured 6.04 mg/mL). Dilute to 1-2 mg/mL final concentration for 3D culture. |
| Source / Origin | Sprague-Dawley (SD) rat tail |
| Application Notes / Precautions | Keep the product cold during handling; warming may cause premature gelation. Because the solution is highly viscous, pipette tips can be trimmed (e.g., 1 mL tips) and the solution mixed by gentle pipetting before use. Preparation of 0.02 M acetic acid: acetic acid density 1.0492 g/mL, molar mass 60.052 g/mol. Required acetic acid mass = 0.02 x 60 = 1.2 g; volume = 1.2 g / 1.0492 g/mL = 1.14 mL. Dilute 1.14 mL glacial acetic acid with water to 1000 mL. Protein concentration varies between batches; for example, one lot measured 6.04 mg/mL, corresponding to 12.08 mg total protein per 2 mL vial. |
| Batch-to-Batch Consistency | Protein concentration varies between batches; refer to the certificate of analysis of each lot for the exact concentration. |
For research use only, not for clinical use.
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