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| Product Name | Collagen Type I Coating Solution, Rat Tail, 3 mg/mL |
| Catalog No. | ATR-CCR-0019 |
| Description | A sterile rat tail collagen type I solution at 3 mg/mL in 0.02 N acetic acid, providing the primary extracellular matrix protein for coating tissue culture surfaces to promote cell attachment and function. |
| Intended Use | Coating tissue culture vessels for the attachment and culture of primary hepatocytes, endothelial cells, muscle cells, and other cell types requiring collagen matrix for optimal growth and differentiation. |
| Principle / Technology | Type I collagen forms fibrillar networks that provide native extracellular matrix structure; cells recognize collagen through integrin alpha-1/beta-1 and alpha-2/beta-1 receptors, initiating attachment, spreading, and tissue-specific gene expression. |
| Detection Method | Cell attachment efficiency, cell morphology and differentiation marker expression by immunocytochemistry |
| Sample Type | Primary hepatocytes, endothelial cells, smooth muscle cells, fibroblasts, embryonic stem cell-derived lineages |
| Performance Range / Specifications | Coating concentration 5–10 µg/cm²; gels can be formed by neutralizing pH and incubating at 37°C for 1 hour |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute in sterile 0.02 N acetic acid or PBS; add to culture surface; incubate 1 hour at room temperature; aspirate; wash 3 times with PBS before seeding cells. |
| Components / Formulation | Rat tail collagen type I 3 mg/mL in 0.02 N acetic acid, sterile-filtered |
| Storage Conditions | 2–8°C; do not freeze |
| Shelf Life | 6 months |
| Package Specifications | 10 mg (3.3 mL), 30 mg (10 mL) |
| Product Form | Liquid, sterile |
| Quality Control | Concentration by hydroxyproline assay, purity by SDS-PAGE, sterility, gel formation and cell attachment activity |
| Key Features | Rat tail origin; high concentration; sterile; consistent gel-forming ability; validated for primary hepatocytes and endothelial cells |
For research use only, not for clinical use.
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