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| Product Name | ClaI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0402 |
| Description | Recognising 5 prime AT^CGAT 3 prime, this rapid recombinant endonuclease cuts plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 20 U/uL, it digests in the unified rapid series buffer at 37°C and is inactivated by heating at 80°C for 20 minutes. Dam and CpG methylation overlapping the site may block cutting, Dcm and EcoKI methylation are tolerated, and EcoBI methylation may influence activity. The enzyme has an exceptionally broad isoschizomer panel including BspDI, BanIII, Bsa29I, BseCI, BshVI, BsiXI, Bsp106I, BspXI, Bsu15I, BsuTUI and ZhoI, easing protocol migration. This unit stores at -20°C |
| Intended Use | Rapid cleavage of plasmid DNA, PCR products and genomic DNA in molecular cloning |
| Principle / Technology | Site specific cleavage at 5 prime AT^CGAT 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dam and CpG methylation may block cleavage; Dcm and EcoKI have no effect; EcoBI may influence cleavage; isoschizomers include BspDI, BanIII, Bsa29I, BseCI, BshVI, BsiXI, Bsp106I, BspXI, Bsu15I, BsuTUI and ZhoI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Single shared buffer across the series; broad isoschizomer compatibility |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Gel electrophoresis of digested DNA |
For in vitro diagnostic use.
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