- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | CHOK1 Human EphA2 (207-976) Stable Cell Line |
| Catalog No. | ATR-CCR-0074 |
| Description | A cryopreserved, adherent CHO-K1 cell line stably expressing c-terminal ha-tagged human EphA2 (207-976). The model is maintained under puromycin selection for cell-based target-binding studies. |
| Intended Use | For FACS binding assays, cell-based ELISA and other target-specific cell research applications. |
| Principle / Technology | Stable transfection followed by antibiotic selection; EphA2 (207-976) expression is qualified by flow cytometry. |
| Detection Method | Flow cytometry; cell-based ELISA |
| Sample Type | Stable mammalian cell line |
| Performance Range / Specifications | Approximately 5 x 10^6 cells in 1 mL per vial; adherent growth. |
| Specificity | Human EphA2 (207-976) |
| Reaction Conditions / Protocol | Culture in F-12K with 10% FBS and 10 ug/mL puromycin. |
| Components / Formulation | Cryopreservation medium: 35% FBS, 55% F-12K and 10% DMSO. |
| Storage Conditions | Store frozen in liquid nitrogen or at -80 C. |
| Package Specifications | 1 vial; approximately 5 x 10^6 cells in 1 mL |
| Product Form | Cryopreserved stable cell line |
| Quality Control | Target expression verified by staining parental and transfected CHO-K1 cells with an anti-EphA2 antibody at 2 ug per 1 x 10^6 cells, followed by a FITC-conjugated secondary antibody and flow-cytometric analysis. |
| Key Features | Stable human EphA2 (207-976) expression; adherent growth; puromycin resistance; c-terminal ha-tagged; qualified for FACS and cell-based ELISA applications. |
| Source / Origin | CHO-K1 cells stably expressing human EphA2 (207-976). |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | FACS binding assays; cell-based ELISA |
| Recommended Buffer System | F-12K with 10% FBS and 10 ug/mL puromycin |
| Application Notes / Precautions | Maintain puromycin selection during routine culture. Keep the vial frozen until cell recovery and use aseptic cell-culture technique. |
| Genotype | Stable human EphA2 (207-976) expression; puromycin-resistant. |
| Alternative Names / Synonyms | EphA2 (207-976); EPHA2; Ephrin type-A receptor 2 |
| Lead Time | 1-3 working days |
| Host Species | Human |
| Tag Information | C-terminal HA-tagged |
| UniProt Accession | P29317 |
| Expression Region | 207-976 aa |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
