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| Product Name | CHOK1 Human CCR8 & CTLA4 Stable Cell Line |
| Catalog No. | ATR-CCR-0065 |
| Description | A cryopreserved, adherent CHO-K1 cell line stably expressing tag-free human CCR8 & CTLA4. The cells are maintained under puromycin and blasticidin selection and are intended for cell-based research assays. |
| Intended Use | For FACS binding assays and cell-based ELISA. |
| Principle / Technology | Stable transfection followed by antibiotic selection; CCR8 & CTLA4 expression is qualified using flow cytometry; cell-based ELISA. |
| Detection Method | Flow cytometry; cell-based ELISA |
| Sample Type | Stable mammalian cell line |
| Performance Range / Specifications | Approximately 5 × 10^6 cells in 1 mL per vial; adherent growth. |
| Specificity | Human CCR8 and CTLA4 |
| Reaction Conditions / Protocol | Culture in F-12K with 10% FBS, 10 μg/mL puromycin and 8 μg/mL blasticidin. |
| Components / Formulation | Cryopreservation medium: 35% FBS, 55% F-12K and 10% DMSO. |
| Storage Conditions | Store frozen in liquid nitrogen or at -80°C. |
| Package Specifications | 1 vial; approximately 5 × 10^6 cells in 1 mL |
| Product Form | Cryopreserved stable cell line |
| Quality Control | CCR8 and CTLA4 co-expression verified by flow cytometry against the parental CHO-K1 cell line. |
| Key Features | Stable human CCR8 & CTLA4 expression; adherent growth; puromycin and blasticidin resistance; tag-free; suitable for FACS binding assays and cell-based ELISA. |
| Source / Origin | CHO-K1 cells expressing human CCR8 & CTLA4 (UniProt P51685 and P16410). |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | FACS binding assays and cell-based ELISA |
| Recommended Buffer System | F-12K with 10% FBS, 10 μg/mL puromycin and 8 μg/mL blasticidin |
| Application Notes / Precautions | Maintain puromycin and blasticidin selection during routine culture. Keep the product frozen until cell recovery. |
| Genotype | Stable human CCR8 and CTLA4 co-expression; puromycin- and blasticidin-resistant. |
| Alternative Names / Synonyms | CCR8 & CTLA4; C-C chemokine receptor type 8 and Cytotoxic T-lymphocyte protein 4 |
| Host Species | Human |
| Tag Information | Tag-Free |
For research use only, not for clinical use.
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