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| Product Name | Chemically Defined Serum-Free Medium for Human Pluripotent Stem Cells with rh bFGF and rh TGF-β |
| Catalog No. | RERE-0019 |
| Description | This two-component medium supports growth and expansion of human pluripotent stem cells and embryonic stem cells in a chemically defined, serum-free, animal-origin-free system. The 10× supplement already contains recombinant human basic fibroblast growth factor and recombinant human transforming growth factor beta, so no additional growth factors need to be sourced or titrated. Prepared with water for injection, the cytokines stay highly active and stable, and one feed sustains the culture beyond 48 hours. The system works under feeder-free conditions in both standard 2D plates and microcarrier-based 3D culture. |
| Intended Use | Growth and expansion of human pluripotent stem cells and embryonic stem cells under feeder-free, chemically defined conditions, in 2D or microcarrier 3D formats. |
| Principle / Technology | A defined basal formulation plus a 10× supplement carrying recombinant human bFGF and TGF-β maintains pluripotency signalling without serum, animal-derived components or extra growth factors. |
| Detection Method | Culture performance is judged by standard hPSC read-outs - morphology, pluripotency marker staining and expansion kinetics run by the laboratory. |
| Sample Type | Human pluripotent stem cells or embryonic stem cells. |
| Performance Range / Specifications | Chemically defined; serum-free; animal-origin-free; rh bFGF and rh TGF-β included in the supplement; prepared with water for injection; single feed sustains culture beyond 48 hours; feeder-free; supports 2D and microcarrier 3D culture; kit: base medium 450 mL plus 10× supplement 50 mL. |
| Specificity | Formulated for human pluripotent and embryonic stem cells; pairs with matrix coatings such as vitronectin, fibronectin or laminin-521 for a complete feeder-free system. |
| Reaction Conditions / Protocol | Dilute the 10× supplement into the basal medium, equilibrate the complete medium, then culture with medium changes at up to 48-hour intervals under feeder-free conditions on the chosen 2D or 3D format. |
| Components / Formulation | Base medium 450 mL (store at 2-8 °C, protected from light) and 10× supplement 50 mL containing rh bFGF and rh TGF-β (store at -25 to -15 °C, protected from light). |
| Storage Conditions | Base medium (component A) at 2-8 °C protected from light; supplement (component B) at -25 to -15 °C protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 450 mL base medium bottle plus 50 mL supplement bottle (500 mL total). |
| Product Form | Liquid basal medium and 10× liquid supplement. |
| Quality Control | Cytokine activity and stability are controlled so that a single feed sustains growth beyond 48 hours. |
| Key Features | - rh bFGF and rh TGF-β already in the supplement - Chemically defined, serum-free and animal-origin-free - Supports 2D plates and microcarrier 3D culture - Single feed sustains the culture beyond 48 hours - Prepared with water for injection |
| Concentration | Supplied as ready-to-use basal medium with a 10× concentrate supplement. |
| Activity / Unit Definition | One kit supports routine hPSC/hESC expansion; performance is defined by sustained growth over 48-hour feeding intervals. |
| Source / Origin | Manufactured from defined raw materials including recombinant human growth factors, prepared with water for injection; animal-origin-free. |
| pH Range / Optimal pH | Does not apply; pH is not a meaningful specification for this product type. |
| Shipping Conditions | Base medium ships with ice packs; the supplement ships on dry ice. |
| Expiration Date / Stability | Remains stable for the stated period provided the cold chain is not broken and light exposure is avoided. |
| Regulatory / Compliance | Restricted to research applications. |
| Compatibility | Pairs with matrix coatings such as vitronectin, fibronectin or laminin-521, and supports both 2D plate culture and microcarrier-based 3D culture. |
| Recommended Buffer System | The basal medium is the working buffer; the 10× supplement is diluted into it before use. |
| Application Notes / Precautions | Keep the two components at their respective storage temperatures and protect both from light. Dilute the supplement into basal medium shortly before use, avoid repeated warm-cold cycling, and hold feeding intervals within the 48-hour window. |
| Batch-to-Batch Consistency | Lot-to-lot consistency is controlled through defined manufacturing specifications; each lot ships with its release record. |
| Model Type | Defined serum-free cell culture medium (two-component system with growth factors) |
| Starting Material / Tissue Site | Chemically defined basal medium plus 10× supplement containing recombinant human bFGF and TGF-β, prepared with water for injection |
| Delivery Standard | 500 mL kit: 450 mL base plus 50 mL 10× supplement |
| Turnaround Time | No turnaround figure is published; shipping is arranged when the order is confirmed. |
| Readouts / Downstream Assays | Standard hPSC culture read-outs run by the user laboratory. |
For research use only, not for clinical use.
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