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| Product Name | Chemically Defined Serum-Free Medium for Human Pluripotent Stem Cells, 8-Supplement Formulation |
| Catalog No. | RERE-0018 |
| Description | This medium supports the growth and expansion of human pluripotent stem cells and embryonic stem cells in a chemically defined, serum-free, animal-origin-free system. Instead of the complex formulations of conventional serum-free media, it adds just eight stem-cell essential components to a straightforward basal formulation, which keeps the culture background clean and the results steady. It is prepared with water for injection, and the cytokines included are highly active and stable, so a single feed sustains the culture for more than 48 hours under feeder-free conditions. |
| Intended Use | Growth and expansion of human pluripotent stem cells (hPSC) and embryonic stem cells (hESC) under chemically defined, feeder-free conditions. |
| Principle / Technology | A defined basal formulation carries eight stem-cell essential supplements, replacing serum and complex additive cocktails; highly stable cytokines maintain pluripotency signalling between feeds. |
| Detection Method | Culture performance is judged by standard hPSC read-outs - morphology, pluripotency marker staining and expansion kinetics run by the laboratory. |
| Sample Type | Human pluripotent stem cells or embryonic stem cells. |
| Performance Range / Specifications | Chemically defined, serum-free, animal-origin-free; eight essential supplements; prepared with water for injection; single feed sustains culture beyond 48 hours; feeder-free use; kit: base medium 450 mL plus 10× supplement 50 mL (500 mL total). |
| Specificity | Formulated for human pluripotent and embryonic stem cells; can be paired with extracellular matrix coatings such as vitronectin, fibronectin or laminin-521 for a complete feeder-free system. |
| Reaction Conditions / Protocol | Dilute the 10× supplement into the basal medium, equilibrate the complete medium, then culture the stem cells with medium changes at up to 48-hour intervals under feeder-free conditions. |
| Components / Formulation | Base medium 450 mL (store at 2-8 °C, protected from light) and 10× supplement 50 mL (store at -25 to -15 °C, protected from light). |
| Storage Conditions | Base medium at 2-8 °C protected from light; supplement at -25 to -15 °C protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 450 mL base medium bottle plus 50 mL supplement bottle (500 mL total). |
| Product Form | Liquid basal medium and 10× liquid supplement. |
| Quality Control | Cytokine activity and stability are controlled so that a single feed sustains growth beyond 48 hours. |
| Key Features | - Only eight stem-cell essential supplements on a clean basal base - Chemically defined, serum-free and animal-origin-free - Single feed sustains the culture beyond 48 hours - Prepared with water for injection - Feeder-free system with matrix-coating options |
| Concentration | Supplied as ready-to-use basal medium with a 10× concentrate supplement. |
| Activity / Unit Definition | One kit supports routine hPSC/hESC expansion; performance is defined by sustained growth over 48-hour feeding intervals. |
| Source / Origin | Manufactured from defined raw materials, prepared with water for injection; animal-origin-free. |
| Shipping Conditions | Base medium ships with ice packs; the supplement ships on dry ice. |
| Expiration Date / Stability | Stable through the stated shelf life when kept protected from light at the recommended storage temperature. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Pairs with matrix coatings such as vitronectin, fibronectin or laminin-521, and supports both 2D plate culture and microcarrier-based 3D culture. |
| Recommended Buffer System | The basal medium is the working buffer; the 10× supplement is diluted into it before use. |
| Application Notes / Precautions | Store the two components at their different temperatures and protect both from light. Mix the supplement into the basal medium just before use, avoid repeated warm-cold cycling of the supplement, and keep feeding intervals within the 48-hour window. |
| Batch-to-Batch Consistency | Consistency between lots is maintained by fixed production controls and release testing against specification. |
| Model Type | Defined serum-free cell culture medium (two-component system) |
| Starting Material / Tissue Site | Chemically defined basal medium with eight stem-cell essential supplements, prepared with water for injection |
| Delivery Standard | 500 mL kit: 450 mL base plus 50 mL 10× supplement |
| Readouts / Downstream Assays | Standard hPSC culture read-outs run by the user laboratory. |
For research use only, not for clinical use.
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