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| Product Name | Cfr42I (SacII) Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0403 |
| Description | This engineered rapid endonuclease cleaves 5 prime CCGC^GG 3 prime, generating a 3 prime overhang, and completes digestion of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. It is supplied at 20 U/uL, operates in the shared rapid series buffer at 37°C, and is heat inactivated at 80°C for 20 minutes. CpG methylation overlapping the site may obstruct cleavage, relevant for methylated templates, and the isoschizomers SacII, KspI, Sfr303I and SgrBI share the same specificity. |
| Intended Use | Rapid DNA fragment preparation from plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime CCGC^GG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation may block cleavage; isoschizomers SacII, KspI, Sfr303I and SgrBI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 60 T |
| Key Features | One shared buffer across the rapid series; activity margin for difficult templates |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Restriction fragment pattern on agarose gel |
For in vitro diagnostic use.
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