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| Product Name | Centrifuge Tubes, 50 mL, Sterile, Conical Bottom |
| Catalog No. | IGM-HMM-0053 |
| Description | Large-volume sterile 50 mL polypropylene centrifuge tubes with conical bottom for high-capacity pelleting and sample processing. Blue printed graduations and extensive frosted writing surface. Tested for centrifugation up to 9,500 x g. |
| Intended Use | High-volume cell pelleting, large-scale bacterial culture harvesting, protein precipitation, density gradient centrifugation, sample storage, and reagent preparation for bioprocessing and clinical laboratory workflows. |
| Principle / Technology | Conical bottom geometry with 30-degree angle efficiently concentrates pellets in tube tip. Threaded closure with integrated seal ring maintains integrity at high RCF. Wide mouth opening facilitates easy pellet access and resuspension. |
| Detection Method | Maximum RCF centrifuge integrity testing; cap seal at rated speed; graduation accuracy by calibrated fill; sterility batch release |
| Sample Type | Large-volume cell cultures (50-500 mL), bacterial pellets from fermentation, urine sediments, ascites fluid, chromatography fractions, density gradient layers |
| Performance Range / Specifications | Graduated volume 5-50 mL at 2.5 mL intervals; max RCF 9,500 x g; temperature range -80 C to 121 C; graduation accuracy +/- 2% |
| Sensitivity / LOD | Endotoxin <0.1 EU/tube; nuclease non-detectable; cytotoxicity negative per USP <87>; bioburden <0.05 CFU/mL |
| Specificity | No extractable organic compounds >1 ppm; no metal ion leaching detected by ICP-MS; PCR inhibitor-free |
| Reaction Conditions / Protocol | Fill tube to desired level; tighten cap firmly; place in appropriate rotor bucket or adapter; counterbalance identically; centrifuge per protocol. |
| Components / Formulation | Medical-grade polypropylene (USP Class VI) tube, HDPE screw cap with thermoplastic elastomer sealing ring, graduations printed with durable non-smear ink |
| Storage Conditions | 15-30 C; store upright in racks; avoid prolonged UV or direct sunlight exposure |
| Shelf Life | 60 months from sterilization date |
| Package Specifications | 25 tubes per rack, 500 tubes per case; bulk non-racked 500 sterile tubes per case |
| Product Form | Sterile 50 mL conical bottom centrifuge tube, individually or bulk packaged, with screw cap |
| Quality Control | Centrifuge integrity at 9,500 x g; sterility to ISO 11137; graduation accuracy; bioburden and endotoxin per lot; cap torque specification |
| Key Features | Large 50 mL capacity; conical bottom for pellet concentration; sterile and ready to use; accurate graduations; large writing patch; RNase/DNase-free; autoclavable |
| Purity | Sterile (SAL 10^-6); DNase/RNase-free certified; non-pyrogenic; non-cytotoxic (ISO 10993-5); heavy metals <1 ppm |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | USP Class VI virgin polypropylene; manufactured in ISO Class 8 cleanroom under ISO 13485 QMS |
| pH Range / Optimal pH | Chemically resistant pH 1-14 for aqueous solutions; resistant to alcohols, alkanes, and most laboratory solvents at room temperature |
| Shipping Conditions | Ambient; racks packed upright in corrugated boxes; tube cap orientation verified to prevent loosening during transit |
| Expiration Date / Stability | 5 years from sterilization date; tube integrity and sterility maintained in unopened original packaging |
| Regulatory / Compliance | ISO 13485:2016; CE IVD marked; ISO 11137 sterilization; USP Class VI biological reactivity; FDA 21 CFR 820 QMS registered |
| Compatibility | Fits all standard 50 mL centrifuge rotors and adapters; compatible with benchtop and floor-model centrifuges; fits standard tube racks for 50 mL conical tubes; suitable for most sample storage systems |
| Recommended Buffer System | Compatible with all biochemical buffers including PBS, Tris, HEPES; resistant to phenol, guanidine thiocyanate, and TRIzol reagent for nucleic acid extraction applications |
| Application Notes / Precautions | Always balance tubes before centrifugation; do not fill beyond 45 mL for optimal pellet formation; tubes at -80 C should be handled carefully as polypropylene becomes more brittle at cryogenic temperatures; check cap tightness after temperature cycling |
| Batch-to-Batch Consistency | Tube body weight variation <1 g across production batches; centrifugation validated at 9,500 x g with 45 mL water fill; cap leak test performed on statistical sample each lot |
For research use only, not for clinical use.
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