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| Product Name | Cell Separation Medium, BR Grade (PVP-Coated Silica Colloid) |
| Catalog No. | RERE-0231 |
| Description | Density-gradient separation medium built on silica particles coated with polyvinylpyrrolidone, supplied at the BR quality grade. On high-speed centrifugation the colloid forms a continuous, highly stable gradient thanks to its low diffusion constant, and the medium stays iso-osmotic and low in viscosity across working densities while remaining non-toxic to cells and sterilisable. It separates cells, subcellular fractions and large viruses above 70S, recovers intact live cells away from damaged cells and debris, and supports plant protoplast, nucleus, chloroplast and mitochondria separation. Preparation calls for mixing nine parts medium with one part 8.5 percent sodium chloride to reach physiological osmolarity, then diluting with physiological saline to the target density; discontinuous gradients layer from high to low density along a wetted tube wall, samples load modestly, and centrifugation around 400 g for 20 to 25 minutes with slow, even acceleration and deceleration gives the separation, with interface or layerwise collection followed by two washes before culture or assay. Keep bottles tightly closed and out of strong light during storage and incubation; unopened stock keeps indefinitely at room temperature and must not freeze. |
| Intended Use | Gentle density-gradient separation of cells, subcellular components, bacteria and large viruses, plus plant protoplast and organelle work |
| Principle / Technology | Low-diffusion PVP-coated silica colloid forms a stable, non-penetrating, non-toxic continuous gradient under modest centrifugal force |
| Specificity | BR grade; PVP-coated silica colloid; low viscosity and physiological osmolarity adjustable; non-toxic and sterilisable; store at RT, never freeze |
| Reaction Conditions / Protocol | Mix 9 parts medium with 1 part 8.5 percent NaCl, dilute to the target density with 0.85 percent saline, build the gradient, load samples at moderate volume and concentration, centrifuge about 400 g for 20 to 25 minutes with slow acceleration, then collect interface or layerwise and wash twice before use |
| Storage Conditions | RT, do not freeze |
| Package Specifications | 100 mL |
| Key Features | Stable continuous gradient; non-toxic to cells; spans cells, organelles and large viruses; BR grade |
For research use only, not for clinical use.
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