- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Catalase Activity Colorimetric Assay Kit (Hydrogen Peroxide Decomposition Method) |
| Catalog No. | ETR-HMM-0084 |
| Description | Colorimetric assay for catalase activity based on the enzyme-catalyzed decomposition of hydrogen peroxide (H₂O₂) to water and oxygen. Residual H₂O₂ after timed reaction with catalase is measured using a colorimetric probe at 520 nm. The kit measures catalase activity across a wide dynamic range in biological samples including cell lysates, tissue homogenates, erythrocytes, and bacterial extracts. |
| Intended Use | Catalase activity measurement in biological samples; oxidative stress biomarker assessment; antioxidant enzyme profiling; evaluation of catalase-deficient cell lines; bacterial catalase screening; food industry catalase inactivation studies. |
| Principle / Technology | Catalase decomposes 2 H₂O₂ → 2 H₂O + O₂ in timed reaction; residual H₂O₂ reacts with chromogenic probe in presence of HRP to produce pink product (520 nm); catalase activity is inversely proportional to residual H₂O₂ absorbance |
| Detection Method | Colorimetric; endpoint at 520 nm; measure residual H₂O₂ after precisely timed catalase reaction (1-5 min) |
| Sample Type | Cell/tissue lysates, erythrocyte lysates, bacterial extracts, purified catalase; 5-50 µL sample; avoid freeze-thaw cycles which inactivate catalase |
| Sensitivity / LOD | LOD 0.01 U/mL catalase; linear range 0.05-10 U/mL; 1 U = decomposition of 1 µmol H₂O₂ per min at 25 °C, pH 7.0 |
| Specificity | Specific for catalase-mediated H₂O₂ decomposition; other peroxidases may consume H₂O₂ at slower rates — include catalase inhibitor (3-aminotriazole or sodium azide) control for specificity |
| Reaction Conditions / Protocol | Add sample to H₂O₂ substrate (50 mM in phosphate buffer); incubate precisely 1-5 min at 25 °C; stop reaction with sodium azide; add chromogenic detection reagent (HRP + probe); incubate 10 min RT; read A520; catalase activity inversely proportional to A520 |
| Components / Formulation | H₂O₂ substrate (50 mM in phosphate buffer, stabilized), chromogenic detection reagent (HRP + colorimetric probe), stop solution (sodium azide 100 mM), catalase positive control (bovine liver catalase, lyophilized, 100 U), assay buffer (sodium phosphate 50 mM, pH 7.0), H₂O₂ standard (for calibration curve) |
| Storage Conditions | 2-8 °C for most components; H₂O₂ solution protected from light; catalase control at -20 °C |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | 100 assays, 200 assays, 500 assays in 96-well format |
| Product Form | Liquid reagents; lyophilized catalase control |
| Key Features | Indirect colorimetric detection at 520 nm — no UV plate reader required; wide dynamic range; H₂O₂ standard for absolute calibration; catalase inhibitor control for specificity; rapid protocol (~20 min total); compatible with tissue, cell, and bacterial samples |
| Purity | H₂O₂ concentration verified; catalase control specific activity >2,000 U/mg protein; probe purity >97% |
| Concentration | As specified per kit; calibrator and substrate concentrations traceable to reference |
| Activity / Unit Definition | One unit defined as the amount of enzyme that catalyzes conversion of 1 µmol substrate per minute under specified conditions |
| Molecular Weight | Varies by target enzyme as documented |
| Source / Origin | Recombinant or purified from natural sources; synthetic chromogenic/fluorogenic substrates |
| pH Range / Optimal pH | pH 6.0–9.0 depending on specific enzyme assay |
| Shipping Conditions | Cold pack 2-8 °C; H₂O₂ protect from light |
| Expiration Date / Stability | 12 months at 2-8 °C; H₂O₂ solution 12 months at 4 °C; catalase control 12 months at -20 °C; discard H₂O₂ solution if bubbles observed (decomposition) |
| Regulatory / Compliance | For laboratory and research use only; RUO; sodium azide toxic — handle with gloves; H₂O₂ is oxidizer; ISO 9001 |
| Compatibility | Compatible with standard microplate readers (absorbance, fluorescence, luminescence modes) |
| Recommended Buffer System | Tris or phosphate buffer optimized per enzyme assay |
| Application Notes / Precautions | Include positive control and inhibitor control in each assay; read within linear range; protect light-sensitive substrates |
| Batch-to-Batch Consistency | Enzyme activity and standard curve linearity within ±15% of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
