Cas9 Nuclease, Codon-Optimized with NLS, from Streptococcus pyogenes

Name: Cas9 Nuclease, Codon-Optimized with NLS, from Streptococcus pyogenes
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Cat.No: IVDRM-0410 Datasheet

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Product Name	Cas9 Nuclease, Codon-Optimized with NLS, from Streptococcus pyogenes
Catalog No.	IVDRM-0410
Description	Sourced from wild type Streptococcus pyogenes, this RNA guided endonuclease specifically cleaves double stranded DNA and, in the presence of a PAM, can also cut single stranded DNA or RNA. The cut falls inside the target sequence, three bases from the NGG PAM region. Codon optimisation and nuclear localisation signal design deliver high editing efficiency, and recombinant expression in Escherichia coli supports reproducible supply. Applications cover gene modification of haematopoietic stem cells, T cells and other cell types as well as molecular diagnostics for pathogen detection.
Intended Use	CRISPR/Cas9 genome editing of haematopoietic stem cells, T cells and other cells; molecular diagnostics and pathogen detection
Principle / Technology	RNA guided endonucleolytic cleavage of double stranded DNA three bases upstream of the NGG PAM
Detection Method	Double stranded DNA target sequences adjacent to NGG PAM motifs
Performance Range / Specifications	In vitro cleavage activity greater than or equal to 98%; endotoxin below 10 EU/mg; host DNA residue below 3 ng/mL; host protein residue below 0.1 ppm; mycoplasma negative
Specificity	Nuclease, exonuclease, RNase and phosphatase activities all negative
Storage Conditions	-25 to -15°C
Shelf Life	1 year
Package Specifications	100 ug; 500 ug; 1 mg
Key Features	Codon optimisation and NLS design for high editing efficiency; quality panel suited to cell editing workflows
Purity	Greater than or equal to 95%
Concentration	10 to 12 mg/mL
Source / Origin	Streptococcus pyogenes wild type, recombinant expression in Escherichia coli, codon optimised with NLS
Readouts / Downstream Assays	In vitro cleavage assay; gene knockout assessment

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For in vitro diagnostic use.