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| Product Name | Bsu36I Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0594 |
| Description | A rapid series restriction endonuclease recognising the interrupted palindrome 5 prime CCTNAGG 3 prime and cutting between the cytosine and thymine to produce a four base 5 prime overhang. It digests plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes and shares the single rapid series digestion buffer, which keeps reaction setup simple and provides activity redundancy for over-digestion or difficult templates. The enzyme runs at 37°C and is heat inactivated by 20 minutes at 80°C. AxyI, Bse21I and Eco81I are isoschizomers. |
| Intended Use | Rapid DNA digestion for cloning and restriction analysis of plasmids, PCR products and genomic DNA |
| Principle / Technology | Cleavage of the interrupted 5 prime CCTNAGG 3 prime palindrome leaving a four base 5 prime overhang |
| Sample Type | Plasmid DNA, PCR products and genomic DNA |
| Specificity | Isoschizomers AxyI, Bse21I and Eco81I; single shared rapid series buffer |
| Reaction Conditions / Protocol | Digest in 1x rapid series buffer at 37°C for 5 to 15 minutes and heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Interrupted palindrome recognition with a short digestion time; shared buffer across the rapid series |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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