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| Product Name | BstXI Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0530 |
| Description | A type IIP restriction enzyme that recognises 5 prime CCA(N)5^NTGG 3 prime and cuts within the spaced recognition sequence, BstXI is used for DNA fragment cloning and vector construction. The enzyme is supplied with an optimised dedicated buffer that lets it perform at its best, and the buffer contains recombinant albumin, which improves the stability of many enzymes. Digestion runs at 37°C with heat inactivation at 80°C for 20 minutes. Dcm methylation affects cutting, while Dam, CpG, EcoKI and EcoBI methylation have no effect. |
| Intended Use | DNA fragment cloning and vector construction |
| Principle / Technology | Site specific cleavage within the spaced 5 prime CCA(N)5^NTGG 3 prime recognition sequence |
| Sample Type | Double stranded DNA containing the spaced recognition sequence |
| Specificity | Dcm methylation affects cleavage; Dam, CpG, EcoKI and EcoBI methylation have no effect |
| Reaction Conditions / Protocol | Digest in 1x dedicated buffer with recombinant albumin at 37°C; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 500 U |
| Key Features | Spaced recognition site within a six base core; optimised buffer with recombinant albumin |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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