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| Product Name | BstEII Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0391 |
| Description | With specificity for the degenerate sequence 5 prime G^GTNACC 3 prime, this rapid recombinant endonuclease processes plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. The enzyme is standardised at 20 U/uL, digests in the shared rapid series buffer at 37°C, and is inactivated by heating at 80°C for 20 minutes. Dam, Dcm and CpG methylation leave activity untouched, whereas EcoKI and EcoBI methylation at overlapping sequences may influence cutting, and BstPI, Eco91I, EcoO65I and PspE are recognised isoschizomers. This unit ships with ice packs and stores at -20°C |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA at degenerate GGTNACC sites |
| Principle / Technology | Site specific cleavage at 5 prime G^GTNACC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dam, Dcm and CpG methylation have no effect; EcoKI and EcoBI methylation may influence cleavage; isoschizomers BstPI, Eco91I, EcoO65I and PspE |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Shared buffer across the rapid series; activity redundancy for excess substrate |
| Concentration | 20 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Restriction digest profile on agarose gel |
For in vitro diagnostic use.
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