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| Product Name | BstBI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0393 |
| Description | This rapid recombinant endonuclease recognises 5 prime TT^CGAA 3 prime and delivers precise digestion of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. Supplied at 20 U/uL, it runs in the common rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. CpG methylation fully overlapping the site blocks cleavage, and the isoschizomers AsuII, Bpu14I, Bsp119I, BspT104I, NspV and SfuI share the same specificity. The unit ships with ice packs and stores at -20°C |
| Intended Use | Rapid precise digestion of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime TT^CGAA 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation fully overlapping the site blocks cleavage; isoschizomers AsuII, Bpu14I, Bsp119I, BspT104I, NspV and SfuI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | One shared buffer across the series; broad isoschizomer compatibility for protocol migration |
| Concentration | 20 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Restriction fragment analysis on agarose gel |
For in vitro diagnostic use.
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