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| Product Name | BsrGI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0394 |
| Description | Engineered for fast DNA processing, this recombinant endonuclease cuts 5 prime T^GTACA 3 prime and handles plasmid linearisation and gene cloning digests within 5 to 15 minutes. It is supplied at 20 U/uL, digests in the shared rapid series buffer at its 37°C optimum, and is inactivated at 80°C for 20 minutes after the reaction. Dam, Dcm, CpG, EcoKI and EcoBI methylation all leave activity unaffected, and BstAUI and Bsp1407I are documented isoschizomers. |
| Intended Use | Vector construction and gene cloning with rapid digestion of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime T^GTACA 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; isoschizomers BstAUI and Bsp1407I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 30 T |
| Key Features | Single shared buffer across the series; complete methylation tolerance for most common systems |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Digestion pattern on agarose gel |
For in vitro diagnostic use.
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