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| Product Name | BspHI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0398 |
| Description | Recognising 5 prime T^CATGA 3 prime, this rapid recombinant endonuclease prepares DNA fragments for cloning and digests plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. The preparation is standardised at 10 U/uL and performs in the common rapid series buffer at 37°C, followed by inactivation at 80°C for 20 minutes. Dam and EcoBI methylation overlapping the site may block cleavage, a consideration for DNA isolated from Dam positive strains, and CciI and PagI are documented isoschizomers. |
| Intended Use | DNA fragment digestion and cloning with rapid cleavage of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime T^CATGA 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dam and EcoBI methylation may block cleavage; isoschizomers CciI and PagI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 30 T |
| Key Features | One shared buffer for the entire rapid series; tolerant of substrate overload |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Restriction digest pattern on agarose gel |
For in vitro diagnostic use.
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