- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | BspEI Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0596 |
| Description | A type IIP restriction enzyme from Bacillus and purified after recombinant expression in E. coli, BspEI recognises and cuts the TCCGGA sequence, producing a four base 5 prime cohesive overhang. It is supplied at 10 U/uL and runs in the dedicated Cut Buffer C at 37°C, with heat inactivation at 80°C for 20 minutes, and it is not compatible with the general rapid digestion buffer. Dam methylation blocks cleavage, CpG methylation reduces it, and Dcm, EcoKI and EcoBI methylation have no effect. One unit completely digests 1 microgram of Dam-minus lambda DNA in a 50 uL reaction in one hour at 37°C. |
| Intended Use | Restriction digestion ahead of DNA fragment cloning |
| Principle / Technology | Cleavage of the TCCGGA site to produce a four base 5 prime cohesive overhang |
| Sample Type | Plasmid and genomic DNA for cloning |
| Specificity | Dam methylation blocks cleavage and CpG methylation reduces it, while Dcm, EcoKI and EcoBI methylation have no effect; incompatible with the general rapid buffer |
| Reaction Conditions / Protocol | Digest in 1x Cut Buffer C at 37°C and heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 500 U |
| Key Features | Cohesive-end products; dedicated Cut Buffer C for BspEI digestion |
| Concentration | 10 U/uL |
| Activity / Unit Definition | 1 U completely digests 1 ug of Dam-minus lambda DNA in 50 uL in 1 hour at 37°C |
| Source / Origin | Bacillus species |
| Recommended Buffer System | 1x Cut Buffer C |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
