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| Product Name | BsmI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0397 |
| Description | This engineered rapid endonuclease recognises 5 prime GAATGC N^ 3 prime and cleaves plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 10 U/uL, it runs in the single buffer shared by the whole rapid series at 37°C, and heat inactivation takes 20 minutes at 80°C EcoBI methylation overlapping the sequence may obstruct cutting, and the specificity is shared by the isoschizomers BsaMI, Mva1269I and PctI. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for molecular cloning |
| Principle / Technology | Site specific cleavage at 5 prime GAATGC(N)^ 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoBI methylation may block cleavage; isoschizomers BsaMI, Mva1269I and PctI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Single shared buffer across the rapid series; activity redundancy for excess substrate or difficult templates |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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