- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | BsmBI Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0527 |
| Description | A type IIS endonuclease that recognises the non-palindromic sequence 5 prime CGTCTC(N)1 3 prime and cuts outside the recognition site, BsmBI is widely used for Golden Gate assembly, where the distal cleavage behaviour of type IIS enzymes enables efficient seamless assembly of multiple DNA fragments, and for fragment cloning and expression vector construction. The enzyme is supplied with an optimised dedicated buffer containing recombinant albumin for improved stability and runs at 55°C, with heat inactivation at 80°C for 20 minutes. CpG methylation fully overlapping the sequence blocks cutting, EcoBI methylation may affect it, and Dam, Dcm and EcoKI methylation have no effect. |
| Intended Use | Golden Gate assembly, DNA fragment cloning and expression vector construction |
| Principle / Technology | Type IIS cleavage outside the non-palindromic 5 prime CGTCTC(N)1 3 prime site at 55°C |
| Sample Type | Plasmid DNA and assembly modules for Golden Gate workflows |
| Specificity | CpG methylation fully overlapping the sequence blocks cleavage; EcoBI methylation may affect it; Dam, Dcm and EcoKI methylation have no effect |
| Reaction Conditions / Protocol | Digest in 1x dedicated buffer with recombinant albumin at 55°C; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 300 U; 1000 U |
| Key Features | Distal type IIS cleavage for seamless assembly; optimised buffer with recombinant albumin |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of assembled or digested DNA |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
