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| Product Name | BsiWI Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0526 |
| Description | A type IIP restriction enzyme that recognises the palindromic site 5 prime C^GTACG 3 prime, BsiWI is supplied with an optimised dedicated reaction buffer that lets the enzyme perform at its best, and the buffer contains recombinant albumin, which improves the stability of many enzymes. Digestion runs at 55°C and is stopped by heat inactivation at 80°C for 20 minutes. PspLI and Pfl23II are recognised isoschizomers, and one unit fully digests 1 microgram of p615 substrate in one hour at 55°C in a 50 microlitre reaction. |
| Intended Use | DNA fragment cloning and vector construction |
| Principle / Technology | Palindromic site specific cleavage at 5 prime C^GTACG 3 prime at 55°C |
| Sample Type | Double stranded DNA containing CGTACG sites |
| Specificity | Isoschizomers PspLI and Pfl23II |
| Reaction Conditions / Protocol | Digest in 1x dedicated buffer with recombinant albumin at 55°C; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 300 U |
| Key Features | Optimised dedicated buffer; recombinant albumin improves enzyme stability |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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