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| Product Name | Bsh1236I (BstUI) Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0399 |
| Description | Cutting the CpG containing site 5 prime CG^CG 3 prime, this rapid recombinant endonuclease is blocked when CpG methylation overlaps the sequence, which also makes it useful for methylation sensitive analysis of genomic DNA. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 20 U/uL, and operates in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. BstUI, BspFNI, BstFNI and AccII are recognised isoschizomers. |
| Intended Use | Rapid digestion and CpG methylation sensitive analysis at CGCG sites for cloning workflows |
| Principle / Technology | Site specific cleavage at 5 prime CG^CG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation blocks cleavage; isoschizomers BstUI, BspFNI, BstFNI and AccII |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Unified buffer across the rapid series; suited to methylation dependent workflows |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Digestion profile on agarose gel |
For in vitro diagnostic use.
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