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Bovine Transferrin (Holo)

Cat.No: RESK-0476 Datasheet

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Product Name Bovine Transferrin (Holo)
Catalog No. RESK-0476
Description Bovine transferrin is a widely used source of bioavailable iron and an important growth-promoting nutrient in cell and tissue culture. This product is prepared from bovine plasma of regulated origin by affinity chromatography, ensuring high purity and protein integrity, with no caprylic acid residue and no heat- or solvent-induced denaturation during extraction. It is manufactured under an ISO quality system to ensure high product safety standards. Bovine transferrin is supplied as a high-purity, heat-treated lyophilized powder in either apo (iron-depleted) or holo (iron-saturated) forms. Holo transferrin continuously releases iron ions and is converted to apo transferrin. Studies have shown that the iron-saturated form of transferrin is the most effective form for promoting cell growth. This product is the holo (iron-saturated) form, derived from bovine plasma, and is of cell culture grade.
Intended Use Used in cell culture applications requiring direct iron ion supplementation to promote cell growth.
Principle / Technology Holo (iron-saturated) transferrin continuously releases iron ions and is gradually converted to apo transferrin, directly supplying iron ions to cells. Prepared from bovine plasma by affinity chromatography.
Performance Range / Specifications English synonyms: Serum transferrin; beta-1-Metal-combining protein; Siderophilin
Source: bovine plasma
Appearance: red lyophilized powder or lump
Molecular weight: approximately 76,000-81,000 Da
Protein content: >=95%
Iron content: >=1200 ug/g
Solubility: soluble in water
Endotoxin: <=5 EU/mg
pH value: 6.0-8.0
Reaction Conditions / Protocol Dissolve before use, for example in PBS or other suitable solvents.
Storage Conditions 2-8 °C
Shelf Life 4 years
Package Specifications 100 mg, 1 g
Product Form Red lyophilized powder or lump
Quality Control Prepared by affinity chromatography from bovine plasma of regulated origin. Manufactured under an ISO quality system; high purity and protein integrity; no caprylic acid residue; no heat- or solvent-induced denaturation during extraction. Endotoxin <=5 EU/mg.
Purity Protein content >=95%
Molecular Weight Approximately 76,000-81,000 Da
Source / Origin Bovine plasma, prepared by affinity chromatography.
pH Range / Optimal pH 6.0-8.0
Regulatory / Compliance For research use only.
Recommended Buffer System Dissolve in PBS or other suitable solvents.
Application Notes / Precautions The holo form is the most effective transferrin form for promoting cell growth and is used when direct iron supplementation is required. Dissolve the lyophilized powder before use, for example in PBS or other suitable solvents.

For research use only, not for clinical use.

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