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| Product Name | Bovine Transferrin (Apo) |
| Catalog No. | RESK-0475 |
| Description | Bovine transferrin is a widely used source of bioavailable iron and an important growth-promoting nutrient in cell and tissue culture. This product is prepared from New Zealand-sourced bovine plasma by affinity chromatography, ensuring high purity and protein integrity, with no caprylic acid residue and no heat- or solvent-induced denaturation during extraction. It is manufactured under an ISO quality system to ensure high product safety standards. Bovine transferrin is supplied as a high-purity, heat-treated lyophilized powder in either apo (iron-depleted) or holo (iron-saturated) forms. Holo transferrin continuously releases iron ions and is converted to apo transferrin. The appropriate form should be selected according to the culture conditions; for example, apo transferrin is generally chosen for media containing high concentrations of iron, such as DMEM/F12. This product is the apo form, with an iron content of |
| Intended Use | Used as a bioavailable iron carrier and growth-promoting nutrient in cell and tissue culture, especially for applications requiring controlled iron ion concentrations. |
| Principle / Technology | Transferrin acts as an iron carrier that binds iron ions. The apo (iron-depleted) form contains minimal bound iron and can take up iron ions from the culture environment, allowing controlled iron availability in cell culture. Prepared from bovine plasma by affinity chromatography. |
| Performance Range / Specifications | Iron content: <=80 ug/g Appearance: off-white lyophilized powder Form: high-purity, heat-treated lyophilized powder |
| Reaction Conditions / Protocol | Dissolve the lyophilized powder before use. Generally, dissolve transferrin lyophilized powder in PBS or pure water at room temperature to prepare a stock solution at 2 mg/mL, then sterilize by filtration through a sterile filter membrane. The filtrate can be stored aseptically at 4 °C for 5-10 days. |
| Storage Conditions | 2-8 °C; keep the lyophilized powder refrigerated and dissolve only before use. |
| Shelf Life | 3 years |
| Package Specifications | 100 mg |
| Product Form | Lyophilized powder (off-white) |
| Quality Control | Prepared by affinity chromatography from New Zealand-sourced bovine plasma. Manufactured under an ISO quality system; high purity and protein integrity; no caprylic acid residue; no heat- or solvent-induced denaturation during extraction. |
| Source / Origin | Bovine plasma (New Zealand-sourced), prepared by affinity chromatography. |
| Regulatory / Compliance | For research use only. |
| Recommended Buffer System | Dissolve in PBS or pure water. |
| Application Notes / Precautions | Select the transferrin form according to the culture conditions: for media with high iron concentrations (e.g., DMEM/F12), apo transferrin is generally recommended. Prepare stock solutions at 2 mg/mL and sterile-filter before use; the filtered solution can be kept aseptically at 4 °C for 5-10 days. |
For research use only, not for clinical use.
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