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| Product Name | BL21 (DE3) Chemically Competent Cells |
| Catalog No. | ATR-CCR-0045 |
| Description | Chemically competent BL21 (DE3) cells for efficient transformation and T7 RNA polymerase-driven prokaryotic protein expression. |
| Intended Use | High-efficiency prokaryotic expression of non-toxic recombinant proteins. |
| Principle / Technology | The DE3 lysogen supplies T7 RNA polymerase for expression from compatible T7 promoter-based vectors. |
| Sample Type | Expression plasmid DNA. |
| Performance Range / Specifications | Transformation efficiency greater than 10⁷ cfu/µg DNA. |
| Specificity | Optimized as a T7-based host for expression of non-toxic proteins. |
| Components / Formulation | Aliquoted chemically competent BL21 (DE3) Escherichia coli cell suspension. |
| Storage Conditions | Store at −85 to −65°C. |
| Package Specifications | 10 × 100 µL; 20 × 100 µL. |
| Product Form | Chemically competent E. coli expression cells. |
| Quality Control | Transformation efficiency is qualified at greater than 10⁷ cfu/µg DNA. |
| Key Features | Defined genetic background; T7 expression system; suitable for high-level expression of non-toxic proteins. |
| Compatibility | Compatible with T7 promoter-based expression vectors, including pET-type systems. |
| Application Notes / Precautions | Keep frozen until use and minimize freeze–thaw cycles. |
| Genotype | F− ompT hsdS(rB− mB−) gal dcm (DE3) |
For research use only, not for clinical use.
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