BglII Rapid Restriction Endonuclease (50 U/uL)
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BglII Rapid Restriction Endonuclease (50 U/uL)

Cat.No: IVDRM-0392 Datasheet

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Product Details Related Products
Product Name BglII Rapid Restriction Endonuclease (50 U/uL)
Catalog No. IVDRM-0392
Description Cutting 5 prime A^GATCT 3 prime, this engineered rapid endonuclease prepares DNA fragments for cloning and digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. It is supplied at a high concentration of 50 U/uL, operates in the unified rapid series buffer at its 37°C optimum, and is heat inactivated at 80°C for 20 minutes. The high concentration format suits workflows where minimal glycerol carryover and concentrated enzyme dosing matter. This unit ships with ice packs and stores at -20°C
Intended Use DNA fragment cloning with rapid digestion of plasmid DNA, PCR products and genomic DNA
Principle / Technology Site specific cleavage at 5 prime A^GATCT 3 prime within 5 to 15 minutes at 37°C
Reaction Conditions / Protocol Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes
Storage Conditions -20°C
Shelf Life 2 years
Package Specifications 100 T
Key Features High concentration 50 U/uL format; single shared buffer across the rapid series
Concentration 50 U/uL
Shipping Conditions Ice packs
Readouts / Downstream Assays Agarose gel profile of digested DNA

For in vitro diagnostic use.

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