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| Product Name | beta1-4 Galactosidase |
| Catalog No. | IVDRM-0308 |
| Description | beta1-4 Galactosidase is a highly specific exo-glycosidase produced by recombinant expression in E. coli from a gene derived from Streptococcus pneumoniae. The enzyme catalyses hydrolysis of non-reducing terminal galactose residues joined through beta1-4 linkages on oligosaccharides, supporting sequential glycan sequencing and structural characterisation of glycoconjugates. |
| Intended Use | Hydrolysis of non-reducing terminal beta1-4 linked galactose residues during glycan sequencing and structural analysis. |
| Principle / Technology | The exo-glycosidase removes terminal beta1-4 linked galactose from oligosaccharide chains, exposing the subterminal sugar for subsequent enzymatic or analytical interrogation. |
| Detection Method | Terminal beta1-4 linked galactose residues |
| Sample Type | Oligosaccharides and glycoconjugates |
| Performance Range / Specifications | Supplied at 8 U per uL with purity above 90 percent by SDS-PAGE; high linkage specificity minimises incidental cleavage of other beta-linked galactose isomers. |
| Specificity | Highly specific for non-reducing terminal beta1-4 linked galactose; does not act on other linkage isomers as stated on the source record. |
| Reaction Conditions / Protocol | Incubate the oligosaccharide substrate with the enzyme in the supplied 10x reaction buffer at 37°C until terminal galactose is released; continue with the next exo-glycosidase in the sequencing sequence or proceed to analysis. |
| Components / Formulation | beta1-4 Galactosidase at 8 U per uL; 10x galactosidase reaction buffer. Volumes scale with the selected pack size. |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 12 months |
| Package Specifications | 400 U or 2000 U packs including matched reaction buffer |
| Product Form | Liquid enzyme supplied with reaction buffer |
| Quality Control | Purity verified by SDS-PAGE (greater than 90 percent); activity confirmed with an AMC-conjugated galactosylated substrate for every lot. |
| Key Features | Strict beta1-4 linkage specificity; recombinant expression in E. coli for consistent performance across lots. |
| Purity | >90% (SDS-PAGE) |
| Concentration | 8 U/uL |
| Activity / Unit Definition | One unit hydrolyses more than 95 percent of the terminal residue from 1 nmol of a Gal-beta1-4 linked AMC substrate within 1 hour at 37°C in a 10 uL reaction. |
| Source / Origin | Recombinant expression in E. coli; gene from Streptococcus pneumoniae |
| pH Range / Optimal pH | 7.5 (storage buffer) |
| Shipping Conditions | Cold pack |
| Expiration Date / Stability | Stable for the full shelf life when held at the recommended storage temperature. |
| Regulatory / Compliance | For research and diagnostic reagent use; manufactured under controlled conditions with lot-specific release testing. |
| Compatibility | Suitable for sequential exo-glycosidase digestion panels and fluorogenic glycan assays. |
| Recommended Buffer System | 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5 |
| Application Notes / Precautions | Confirm that the target galactose is terminal and beta1-4 linked; internal or beta1-3/1-6 linked galactose is not a substrate. |
| Batch-to-Batch Consistency | Each lot is released with activity and purity documentation; lot number is printed on the vial label. |
| Delivery Standard | Cold pack dispatch by default |
| Turnaround Time | Standard dispatch |
| Readouts / Downstream Assays | SDS-PAGE, fluorogenic glycosidase assays, glycan sequencing workflows |
For in vitro diagnostic use.
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