- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | beta-N-Acetylhexosaminidase |
| Catalog No. | IVDRM-0309 |
| Description | beta-N-Acetylhexosaminidase is an important tool enzyme for glycosylation research, produced by recombinant expression in E. coli from a gene derived from Streptomyces plicatus. It catalyses hydrolysis of non-reducing terminal N-acetylglucosamine joined through beta 1-3, 1-4 and 1-6 linkages as well as N-acetylgalactosamine joined through beta 1-4 linkages, making it a versatile reagent for trimming terminal hexosamines from oligosaccharide chains. |
| Intended Use | Hydrolysis of terminal beta-linked N-acetylglucosamine and N-acetylgalactosamine residues in glycosylation studies and deglycosylation experiments. |
| Principle / Technology | The enzyme cleaves terminal beta-linked hexosamine residues from the non-reducing end of oligosaccharides, exposing the underlying saccharide for further analysis or digestion. |
| Detection Method | Terminal beta-linked GlcNAc and GalNAc residues |
| Sample Type | Oligosaccharides and glycoconjugates |
| Performance Range / Specifications | Supplied at 5 U per uL with purity above 90 percent by SDS-PAGE; activity confirmed with an AMC-conjugated hexosaminidase substrate before release. |
| Specificity | Acts on beta 1-3, 1-4 and 1-6 linked terminal GlcNAc and beta 1-4 linked terminal GalNAc residues. |
| Reaction Conditions / Protocol | Incubate the substrate with the enzyme in the supplied 10x reaction buffer at 37°C until terminal hexosamine residues are released; proceed to the next digestion step or analytical method. |
| Components / Formulation | beta-N-Acetylhexosaminidase at 5 U per uL; 10x hexosaminidase reaction buffer. Volumes scale with the selected pack size. |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 24 months |
| Package Specifications | 500 U packs including matched reaction buffer |
| Product Form | Liquid enzyme supplied with reaction buffer |
| Quality Control | Purity verified by SDS-PAGE (greater than 90 percent); activity confirmed with an AMC-conjugated substrate for every lot. |
| Key Features | Broad terminal hexosamine coverage spanning multiple beta linkage types; recombinant expression for lot-to-lot reproducibility. |
| Purity | >90% (SDS-PAGE) |
| Concentration | 5 U/uL |
| Activity / Unit Definition | One unit hydrolyses more than 95 percent of terminal beta-D-N-acetylgalactosamine from 1 nmol of a GalNAc-beta1-4 linked AMC substrate within 1 hour at 37°C in a 10 uL reaction. |
| Source / Origin | Recombinant expression in E. coli; gene from Streptomyces plicatus |
| pH Range / Optimal pH | 7.5 (storage buffer) |
| Shipping Conditions | Cold pack |
| Expiration Date / Stability | Stable for the full shelf life when held at the recommended storage temperature. |
| Regulatory / Compliance | For research and diagnostic reagent use; manufactured under controlled conditions with lot-specific release testing. |
| Compatibility | Suitable for sequential exo-glycosidase digestion panels and glycan structural workflows. |
| Recommended Buffer System | 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5 |
| Application Notes / Precautions | Substrates must present terminal beta-linked hexosamine; confirm linkage type before designing the digestion panel. |
| Batch-to-Batch Consistency | Each lot is released with activity and purity documentation; lot number is printed on the vial label. |
| Delivery Standard | Cold pack dispatch by default |
| Turnaround Time | Standard dispatch |
| Readouts / Downstream Assays | SDS-PAGE, fluorogenic glycosidase assays, glycan sequencing workflows |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
