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| Product Name | BclI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0389 |
| Description | This rapid recombinant endonuclease targets 5 prime T^GATCA 3 prime and cleaves plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes, serving DNA fragmentation and cloning as well as gene editing sample preparation. It is supplied at 20 U/uL, digests in the common rapid series buffer at 37°C, and is heat inactivated at 80°C for 20 minutes. Dam and EcoBI methylation fully overlapping the site block cleavage while Dcm, CpG and EcoKI methylation are tolerated. No star activity appears during 3 hours of incubation, though prolonged digestion beyond the recommended window may induce it, and BsiQI, FbaI and Ksp22I are listed isoschizomers. |
| Intended Use | DNA fragmentation for cloning and gene editing sample preparation with rapid digestion |
| Principle / Technology | Site specific cleavage at 5 prime T^GATCA 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dam and EcoBI methylation fully overlapping the site block cleavage; Dcm, CpG and EcoKI have no effect; no star activity within 3 hours; isoschizomers BsiQI, FbaI and Ksp22I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 125 T |
| Key Features | Single shared buffer across the series; predictable star activity profile for long protocols |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Digestion fragment pattern on agarose gel |
For in vitro diagnostic use.
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