- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Basement Membrane Matrix, Phenol Red-Free, LDEV-Free |
| Catalog No. | RESK-0239 |
| Description | This basement membrane matrix is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components include laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and entactin, along with growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other growth factors naturally present in the EHS tumor. At room temperature, the matrix polymerizes to form a biologically active three-dimensional matrix that mimics the structure, composition, physical properties, and function of the in vivo cell basement membrane, supporting in vitro cell culture and differentiation and enabling studies of cell morphology, biochemical function, migration, invasion, and gene expression. The product is sterile and free of phenol red, making it especially suitable for color-sensitive detection such as fluorescence-based assays. Protein concentration is 8-12 mg/mL. |
| Intended Use | For research use in 2D and 3D cell culture, cell invasion and migration assays, in vivo tumorigenesis studies, and applications requiring color-based detection such as fluorescence assays. For research use only. |
| Principle / Technology | At room temperature the preparation polymerizes into a biologically active three-dimensional matrix that mimics the in vivo basement membrane. The resulting 3D culture substrate promotes the attachment and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells, and can modulate gene expression in 3D cultures of adult rat hepatocytes and human mammary epithelial cells. It also serves as a basic scaffold for tumor cell invasion studies, an essential matrix for in vitro and in vivo angiogenesis research, a 3D scaffold for transplanted tumor cell growth in animal models, and supports peripheral nerve regeneration and differentiation of bovine oviduct epithelial cells. |
| Detection Method | Compatible with color-based detection methods, including fluorescence-based assays (phenol red-free formulation avoids interference). |
| Performance Range / Specifications | Sterile: rigorously dialyzed and sterilized; no bacterial or fungal growth Protein concentration: 8-20 mg/mL Endotoxin: <8 EU/mL LDEV-free (no lactate dehydrogenase-elevating virus residue) Mycoplasma-free (verified by testing) Phenol red-free |
| Reaction Conditions / Protocol | Polymerizes at room temperature to form a biologically active three-dimensional matrix. Handle according to standard basement membrane matrix coating and 3D culture procedures. |
| Components / Formulation | Soluble basement membrane preparation extracted from EHS mouse tumor. Main components: laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and entactin; contains growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other factors naturally present in the EHS tumor. Free of phenol red. |
| Storage Conditions | Store at -20 °C. Storage at -80 °C is also acceptable. |
| Shelf Life | 2 years when stored at -20 °C. |
| Package Specifications | 5 mL 10 mL |
| Product Form | Liquid (sterile solution; gels upon polymerization at room temperature) |
| Quality Control | Sterility (no bacterial or fungal growth), protein concentration, endotoxin (<8 EU/mL), LDEV-free, and mycoplasma-free testing are performed. Monthly production capacity exceeds 50 L per batch to ensure stable supply. |
| Concentration | 8-12 mg/mL (lot-specific protein concentration range: 8-20 mg/mL) |
| Source / Origin | EHS (Engelbreth-Holm-Swarm) mouse tumor rich in extracellular matrix proteins |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 2 years at -20 °C; may also be stored at -80 °C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for 2D and 3D culture, invasion and migration assays, in vivo tumorigenesis, and color-based detection such as fluorescence assays. |
| Application Notes / Precautions | Phenol red-free formulation is recommended for assays requiring color-based detection, such as fluorescence detection. LDEV (lactate dehydrogenase-elevating virus) is an RNA virus that infects mouse macrophages and is a common contaminant of biological materials derived from transplantable tumor cell lines; this product is verified to be free of LDEV. |
| Batch-to-Batch Consistency | Large-scale production (monthly batch output at the 50 L level or above) supports consistent supply. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
