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| Product Name | Basement Membrane Matrix, LDEV-Free |
| Catalog No. | RESK-0215 |
| Description | Basement Membrane Matrix, LDEV-Free is a soluble basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, which is rich in extracellular matrix proteins. Its major components are laminin, collagen type IV, heparan sulfate proteoglycan (HSPG) and entactin/nidogen, and it also contains growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors naturally present in the EHS tumor. Under physiological conditions it polymerizes into a biologically active three-dimensional matrix that mimics the structure, composition, physical properties and function of the in vivo cell basement membrane, facilitating cell culture and differentiation in vitro and supporting studies of cell morphology, biochemical function, migration, invasion and gene expression. The 3D matrix formed by this product promotes the attachment and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells and hair follicle cells, and can influence gene expression in three-dimensional cultures of adult mouse hepatocytes and human mammary epithelial cells. It also serves as a basic scaffold for tumor cell invasion studies, an essential matrix for in vitro and in vivo angiogenesis research, a 3D scaffold for transplanted tumor cell growth in animal models, and supports peripheral nerve regeneration and bovine oviduct epithelial cell differentiation. The product is sterile, with a protein concentration of 8-12 mg/mL, and is suitable for a wide range of applications including subcutaneous tumor formation and tumor cell invasion assays. This version contains phenol red. |
| Intended Use | Suitable for 2D and 3D cell culture, cell invasion and migration assays, angiogenesis studies, organoid culture, and in vivo tumorigenesis (subcutaneous tumor formation) experiments. For research use only. |
| Principle / Technology | A soluble basement membrane extract that polymerizes at physiological temperature into a biologically active 3D hydrogel, reconstituting the structural and signaling environment of the natural basement membrane to support cell attachment, differentiation and morphogenesis. |
| Reaction Conditions / Protocol | Thaw on ice or at 4 °C; the matrix is liquid at low temperature and polymerizes into a gel at 37 °C (polymerization begins at room temperature). Working concentration guidelines: Gel formation (gel assays): final concentration must be greater than 3 mg/mL Coating (no gelation required): below 3 mg/mL; optimize by pre-experiment for the specific cell type Invasion assays: 1-2 mg/mL recommended, then optimize for the specific system Angiogenesis assays: use a matrix product with concentration above 10 mg/mL In vivo subcutaneous injection: final concentration above 4 mg/mL to ensure complete gelation 3D culture: final concentration above 4 mg/mL |
| Components / Formulation | Laminin Collagen type IV Heparan sulfate proteoglycan (HSPG) Entactin/nidogen Growth factors: TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other EHS tumor-derived growth factors Contains phenol red |
| Storage Conditions | Store at -20 °C. Storage at -80 °C is also acceptable. |
| Shelf Life | 2 years when stored at -20 °C. |
| Package Specifications | 1.5 mL 5 mL 10 mL |
| Product Form | Liquid (at low temperature); forms a gel upon warming to 37 °C. Contains phenol red. |
| Quality Control | Sterilized by stringent dialysis; verified free of bacterial and fungal growth LDEV-free (free of lactate dehydrogenase-elevating virus, a common contaminant of transplantable tumor-derived biomaterials that infects mouse macrophages and causes persistent infection in laboratory mouse populations) Mycoplasma-free verified by testing Endotoxin: < 8 EU/mL Protein concentration tested per lot |
| Concentration | 8-12 mg/mL protein (per product description; lot-dependent, within an overall range of 8-20 mg/mL) |
| Source / Origin | Extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 2 years at -20 °C; storage at -80 °C is also acceptable. |
| Compatibility | Compatible with standard cell culture media for 2D/3D culture, invasion/migration assays, angiogenesis assays and in vivo tumorigenesis models. |
| Application Notes / Precautions | Keep the matrix cold during handling to prevent premature gelation. For gel-based assays, ensure the final concentration is above 3 mg/mL; coating applications do not require gelation and should use concentrations below 3 mg/mL. For in vivo experiments, use a final concentration above 4 mg/mL to avoid incomplete gelation. This product contains phenol red; a phenol red-free version is available for sensitive applications. LDEV is an RNA virus that alters mouse physiology; this product is verified LDEV-free. |
| Batch-to-Batch Consistency | Large-scale production (monthly output above 50 L) with per-lot protein concentration testing to ensure consistency. |
For research use only, not for clinical use.
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